PCM1 antibody
Principal name
PCM1 antibody
Alternative names for PCM1 antibody
PCM-1, Pericentriolar material 1 protein
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PCM1 antibody
- Kit Component:
- - KN312957G1, Pcm1 gRNA vector 1 in pCas-Guide vector
- KN312957G2, Pcm1 gRNA vector 2 in pCas-Guide vector
- KN312957D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of PCM1 expression in transfected 293T cell line (H00005108-T01) by PCM1 MaxPab polyclonal antibody.Lane 1: PCM1 transfected lysate(58.3 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of PCM-1 antibody (Center)(Cat.#TA324939) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of A549 cell stained with PCM-1 Antibody (Center)(Cat#TA324939/SA120711BC).A549 cells were incubated with PCM-1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .PCM-1 immunoreactivity is localized to Centrosome significantly.
PCM-1 Antibody (Center) (Cat. #TA324939) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using PCM1 antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324939 | PCM1 antibody |
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| Rabbit polyclonal PCM-1 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
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| TA332849 | PCM1 antibody |
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| Rabbit anti-PCM1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
