PCYT1A antibody
Principal name
PCYT1A antibody
Alternative names for PCYT1A antibody
CTPCT, PCYT1, CCT-alpha, CCT A, Choline-phosphate cytidylyltransferase A, CTP:phosphocholine cytidylyltransferase A, Phosphorylcholine transferase A
Ncbi ID
9606, NM_005017, NP_005008, NP_001156631.1, NM_009981, NM_001163159, NM_001163160, NM_078622
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PCYT1A antibody
- Kit Component:
- - KN312985G1, Pcyt1a gRNA vector 1 in pCas-Guide vector
- KN312985G2, Pcyt1a gRNA vector 2 in pCas-Guide vector
- KN312985D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Mostowska, A., et al. J. Med. Genet. (2010) In press
Mostowska, A., et al. Eur. J. Oral Sci. 118(4):325-332(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Western Blot analysis of PCYT1A expression in transfected 293T cell line by PCYT1A monoclonal antibody (M03), clone 7H8.Lane 1: PCYT1A transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
PCYT1A monoclonal antibody (M03), clone 7H8 Western Blot analysis of PCYT1A expression in K-562 ( Cat # L009V1 ).
PCYT1A monoclonal antibody (M03), clone 7H8. Western Blot analysis of PCYT1A expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged PCYT1A is approximately 0.3ng/ml as a capture antibody.
PCYT1A monoclonal antibody (M02), clone 6E6. Western Blot analysis of PCYT1A expression in PC-12 ( Cat # L012V1 ).
PCYT1A monoclonal antibody (M02), clone 6E6. Western Blot analysis of PCYT1A expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged PCYT1A is approximately 3ng/ml as a capture antibody.
PCYT1A MaxPab rabbit polyclonal antibody. Western Blot analysis of PCYT1A expression in mouse intestine.
Western Blot analysis of PCYT1A expression in transfected 293T cell line (H00005130-T02) by PCYT1A MaxPab polyclonal antibody.Lane 1: PCYT1A transfected lysate(41.70 KDa).Lane 2: Non-transfected lysate.
PCYT1A Antibody (N-term) (Cat. #TA324475) western blot analysis in K562,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PCYT1A antibody detected the PCYT1A protein (arrow).
PCYT1A Antibody (N-term) (Cat. #TA324475)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCYT1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PCYT1A Antibody (N-term)(Cat#TA324475) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324475 | PCYT1A (N-term) antibody |
||||||
| Rabbit polyclonal PCYT1A Antibody (N-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308130 | PCYT1A |
||||
| Recombinant protein of human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417575 | PCYT1A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205644L2V | Lenti ORF particles, Pcyt1a (GFP-tagged) - Mouse phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205644L1V | Lenti ORF particles, Pcyt1a (Myc-DDK-tagged) - Mouse phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214938L2V | Lenti ORF particles, Pcyt1a (GFP-tagged ORF) - Rat phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR214938L1V | Lenti ORF particles, Pcyt1a (Myc-DDK-tagged ORF) - Rat phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||