PDE12 antibody
Principal name
PDE12 antibody
Alternative names for PDE12 antibody
2'-PDE, 2-PDE, 2',5'-phosphodiesterase 12
Ncbi ID
NP_808881, ACE87622.1, NP_808881.3, NP_848783.3, NM_178668, NM_177966
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of PDE12 antibody
- Kit Component:
- - KN313007G1, E430028B21Rik gRNA vector 1 in pCas-Guide vector
- KN313007G2, E430028B21Rik gRNA vector 2 in pCas-Guide vector
- KN313007D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kohno, T., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 5), 520-522 (2010)
Kubota, K., et al. J. Biol. Chem. 279(36):37832-37841(2004)
Human OVCAR-3; WB Suggested Anti-PDE12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate. PDE12 is supported by BioGPS gene expression data to be expressed in OVCAR3.; PDE12 antibody - middle region (ARP53350_P050) in Human OVCAR-3 cells using Western Blot
Western blot analysis of PDE12 Antibody (C-term) Cat.-No AP53232PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the PDE12 antibody detected the PDE12 protein (arrow).
Flow cytometric analysis of MDA-MB231 cells using PDE12 Antibody (C-term) Cat.-No AP53232PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-PDE12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: OVCAR-3 cell lysatePDE12 is supported by BioGPS gene expression data to be expressed in OVCAR3
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP53232PU-N | PDE12 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317016 | PDE12 |
||||
| Recombinant protein of human phosphodiesterase 12 (PDE12) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209382L2V | Lenti ORF particles, Pde12 (GFP-tagged) - Mouse phosphodiesterase 12 (Pde12), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,340.00
|
|
| OriGene Technologies, Inc. | ||
| MR209382L1V | Lenti ORF particles, Pde12 (Myc-DDK-tagged) - Mouse phosphodiesterase 12 (Pde12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
