PDE2A antibody
Principal name
PDE2A antibody
Alternative names for PDE2A antibody
CGS-PDE, cGSPDE, cGMP-dependent 3',5'-cyclic phosphodiesterase, Cyclic GMP-stimulated phosphodiesterase
Ncbi ID
9606, NP_001137311.1, NP_002590, NM_001143847, NM_031079, NM_002599, NM_001143839, NM_001146209
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PDE2A antibody
Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 2 There are two member of PDE2A gene are cloned and designated as PD2A and PDE2B (Rascon et. al., 2002). Each of the PDE2A and PDE2B genes are 2793 Bases and 930 amino acids with an apparent MW of 100 kDA. The catalytic domain of PDE2A is homologous to all other 11 PDE family members. The PDE2A has specificity for cAMP with Km of 2.4 uM and its activity is modulated by the presence of cGMP. In contrast, the PDE2B does not hydrolyze cGMP nor its activity is altered by the presence of cGMP. Western blot analyses of PDE2A reveals a variety of tissues including neocortex, cerebellum, heart, kidney lungs, pulmonary artery and skeletal muscle (Sadhu et. al., 2002). The PDE2A expression was evident in venous arterial endothelial cells but not in arterial endothelial cells (Sadhu et. al., 1999). The PDE2A expression was also noted in corpus cavernosum along with a wide repertoire of other PDEs enzymes (Kuthe et. al., 1999).
PDE2A monoclonal antibody (M01), clone 4F4 Western Blot analysis of PDE2A expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged PDE2A is approximately 0.03ng/ml as a capture antibody.
Western blot using AP00652SU-N (1:20,000) against lysate from COS cells transfected with the indicated human PDE isoform.
Immunostaining of PDE2A expressing cells with AP10205PU-N (1:250).
Rat Brain; WB Suggested Anti-Pde2a Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Brain; Pde2a antibody - N-terminal region (ARP56379_P050) in Rat Brain cells using Western Blot
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PDE2A monoclonal antibody. (1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502318), and then analyzed by flow cytometry.
Anti-PDE2A mouse monoclonal antibody (TA502319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A(RC207219).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502319), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502320)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502320)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502320)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PDE2A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502320), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502321), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PDE2A antibody(TA502321),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody(TA502321),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-PDE2A mouse monoclonal antibody (TA502349) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A(RC207219).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502349), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502353), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502354), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502389)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502389)
Anti-PDE2A mouse monoclonal antibody (TA502389) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A(RC207219).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody(TA502389),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502389), and then analyzed by flow cytometry.
Anti-PDE2A mouse monoclonal antibody (TA502683) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A(RC207219).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
HEK293T cells transfected with either RC207219 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE2A antibody(TA502683), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PDE2A antibody(TA502683),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody(TA502683),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-PDE2A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB Suggested Anti-Pde2a Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Brain
- Quick-Filters:
- Cited (1)
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502320 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI1G9 (formerly 1G9) | |||||||
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Mouse | IgG1 | OTI1G9 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Published recently! Highly validated antibody!
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| TA502321 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI4H8 (formerly 4H8) | |||||||
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Mouse | IgG1 | OTI4H8 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
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| TA502389 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI3A4 (formerly 3A4) | |||||||
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Mouse | IgG2b | OTI3A4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
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| TA502683 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI4D10 (formerly 4D10) | |||||||
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Mouse | IgG2a | OTI4D10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
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| TA341106 | PDE2A (N-term) antibody |
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| Rabbit Polyclonal Anti-PDE2A Antibody (N-Terminus) | |||||||
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Rabbit | Aff - Purified | Hu, Rt | P |
50 µg /
€425.00
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| OriGene Technologies, Inc. | |||||||
| TA502318 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI5D9 (formerly 5D9) | |||||||
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Mouse | IgG1 | OTI5D9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA502319 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI5B10 (formerly 5B10) | |||||||
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Mouse | IgG2a | OTI5B10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA502349 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
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Mouse | IgG1 | OTI2A5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA502353 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI4D2 (formerly 4D2) | |||||||
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Mouse | IgG2a | OTI4D2 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA502354 | PDE2A antibody |
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| PDE2A mouse monoclonal antibody, clone OTI2F1 (formerly 2F1) | |||||||
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Mouse | IgG1 | OTI2F1 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP00652SU-N | PDE2A antibody |
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Rabbit | Serum | Hu, Ms, Rt | C, IP, WB |
0.1 ml /
€440.00
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| OriGene Technologies GmbH | |||||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC226806L2V | Lenti ORF particles, PDE2A (mGFP-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€2,050.00
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| OriGene Technologies, Inc. | ||
| RC228618L2V | Lenti ORF particles, PDE2A (mGFP-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4, 200 uL, >10^7 TU/mL |
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200 µl /
€2,050.00
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200272L2V | Lenti ORF particles, Pde2a (GFP-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,790.00
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| OriGene Technologies, Inc. | ||
| RR200273L2V | Lenti ORF particles, Pde2a (GFP-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€1,780.00
|
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| OriGene Technologies, Inc. | ||
| RR200272L1V | Lenti ORF particles, Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,620.00
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| OriGene Technologies, Inc. | ||
| RR200273L1V | Lenti ORF particles, Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€1,610.00
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| OriGene Technologies, Inc. | ||