PDE4C antibody

Background of PDE4C antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. cAMP-specific phosphodiesterase type-4 (PDE4) family is comprised of 4 genes (PDE4 A, B, C and D) with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1). The cAMP-dependent protein kinase A modulates the enzyme activity of some PDE4 family members by phosphorylation/dephosphorylation mechanisms (2, 3). Such short-term regulatory mechanisms are necessary for rapid changes in the cAMP levels in cells. PDE4-family selective inhibitor, rolipram inhibits all members of the PDE4 family in mM range. At present PDE4 subtype selective and variant selective inhibitors are not available. In rodents inhibition of PDE4 enzymes by rolipram attenuated short- and long-term memory impairment produced by the administration of scopolamine and MK801 (3, 4). Interaction of these enzymes with cellular scaffolding proteins plays important role in PDE4 compartmentalization and cAMP-dependent signaling. Members of the PDE4A and D subtype variants specifically interact with cytoskeletal proteins with SH3 domains or RACK and AKAP for cellular compartmentalization. In brain PDE4A, B and D are associated with GPCRs signaling (5, 6). Little expression of PDE4C was observed in brain, the PDE4C is expressed in other peripheral tissues including testis, white blood cells and heart.