PDE8A antibody

Background of PDE8A antibody

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP in to AMP are phosphodiesterase type 8. The cAMP-specific phosphodiesterase type-8 (PDE8) family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1). PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. Like other PDEs, human PDE8A has 713 amino acids and migrate at apparent 100 kDa on reduced and non-reduced SDS-PAGE. The PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy-terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) with in the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus.