PDGFD antibody
Principal name
PDGFD antibody
Alternative names for PDGFD antibody
PDGF-D, Platelet-derived growth factor D, SCDGFB, SCDGF-B, IEGF, Iris-expressed growth factor, Spinal cord-derived growth factor B
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)
Background of PDGFD antibody
- Kit Component:
- - KN205520G1, PDGFD gRNA vector 1 in pCas-Guide vector
- KN205520G2, PDGFD gRNA vector 2 in pCas-Guide vector
- KN205520D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bergsten E A., et al. (2001) Nat. Cell Biol. 3:512-516Uutela M., et al. (2004) Blood. 104:3198-3204
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDGFD polyclonal antibody ( Cat # PAB4843 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western Blot analysis of PDGFD expression in transfected 293T cell line (H00080310-T02) by PDGFD MaxPab polyclonal antibody.Lane 1: PDGFD transfected lysate(42.20 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PDGFD transfected lysate using anti-PDGFD MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PDGFD MaxPab mouse polyclonal antibody (B01) (H00080310-B01).
Human Heart; WB Suggested Anti-PDGFD Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; PDGFD antibody - N-terminal region (ARP58814_P050) in Human Heart cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFD (RC219641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFD.
HEK293T cells transfected with either RC219641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDGFD antibody(TA503700), and then analyzed by flow cytometry.
WB Suggested Anti-PDGFD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503700 | PDGFD antibody |
||||||
| PDGFD mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
 |
Mouse | IgG1 | OTI1C1 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760442 | PDGFD (N-term HIS tag, transcript variant 1) |
||||
| Purified recombinant protein of Human platelet derived growth factor D (PDGFD), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403061 | PDGFD overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY409700 | PDGFD overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203421L2V | Lenti ORF particles, Pdgfd (GFP-tagged) - Mouse platelet-derived growth factor, D polypeptide (cDNA clone MGC:31518 IMAGE:4489485), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR203421L1V | Lenti ORF particles, Pdgfd (Myc-DDK-tagged) - Mouse platelet-derived growth factor, D polypeptide (cDNA clone MGC:31518 IMAGE:4489485), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202180L2V | Lenti ORF particles, Pdgfd (GFP-tagged ORF) - Rat platelet-derived growth factor, D polypeptide (Pdgfd), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR202180L1V | Lenti ORF particles, Pdgfd (Myc-DDK-tagged ORF) - Rat platelet-derived growth factor, D polypeptide (Pdgfd), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||