PDHA1 antibody
Principal name
PDHA1 antibody
Alternative names for PDHA1 antibody
PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial
SwissProt ID
A7MB35 (Bovin), P08559 (Human), P16387 (Yeast), P26284 (Rat), P29804 (Pig), P35486 (Mouse), P49823 (Canfa), P52899 (Caeel), Q10489 (Schpo)
Ncbi ID
9606, NP_000275, NP_000275.1, NM_001173456, NM_001173455, NM_001173454, NP_032836.1, NM_000284
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of PDHA1 antibody
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT); and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Glushakova, L.G., et al. Mol. Genet. Metab. 98(3):289-299(2009)
Joao Silva, M., et al. Eur. J. Pediatr. 168(1):17-22(2009)
Boichard, A., et al. Mol. Genet. Metab. 93(3):323-330(2008)
Immunoperoxidase of purified MaxPab antibody to PDHA1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml]
PDHA1 MaxPab polyclonal antibody. Western Blot analysis of PDHA1 expression in human liver.
Western Blot analysis of PDHA1 expression in transfected 293T cell line (H00005160-T01) by PDHA1 MaxPab polyclonal antibody.Lane 1: PDHA1 transfected lysate(43.01 KDa).Lane 2: Non-transfected lysate.
PDHA1 MaxPab polyclonal antibody. Western Blot analysis of PDHA1 expression in HepG2.
mouse brain extract; WB Suggested Anti-PDHA1 Antibody . . Positive Control: Lane 1: 80ug mouse brain extract . Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; PDHA1 antibody - N-terminal region (ARP48136_T100) in mouse brain extract cells using Western Blot
Human HepG2; WB Suggested Anti-PDHA1 Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate. PDHA1 is supported by BioGPS gene expression data to be expressed in HepG2.; PDHA1 antibody - N-terminal region (ARP48136_T100) in Human HepG2 cells using Western Blot
human NT2; WB Suggested Anti-PDHA1 Antibody . . Positive Control: Lane 1: 60ug human NT2 cell line . Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; PDHA1 antibody - C-terminal region (ARP48137_T100) in human NT2 cells using Western Blot
Western blot analysis of PDHA1 in an in vitro autophosphorylation of PDH complex in response to different stimulants with PDHA1 (phospho S293) polyclonal antibody ( Cat # PAB12079 ).Such stimulants are : ( - ) none, H2O2-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator.Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.
Western blot analysis of PDHA1 (arrow) using PDHA1 Antibody Cat.-No AP53240PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.
Western blot analysis of PDHA1 Antibody Cat.-No AP53240PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the PDHA1 antibody detected the PDHA1 protein (arrow).
Western blot analysis of PDHA1 Antibody (Center) Cat.-No AP53241PU-N in Hela cell line lysates (35ug/lane). PDHA1 (arrow) was detected using the purified Pab.
Anti-PDHA1 mouse monoclonal antibody (TA502696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (RC201831, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.
Flow cytometric Analysis of Hela cells, using anti-PDHA1 antibody(TA502696),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PDHA1 antibody(TA502696),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-PDHA1 mouse monoclonal antibody (TA502704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (RC201831, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.
HEK293T cells transfected with either RC201831 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDHA1 antibody(TA502704), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PDHA1 antibody(TA502704),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308687 diluted at 1:5000.
Sample (50 ug of whole cell lysate). A: Rat brain. 10% SDS PAGE. TA308687 diluted at 1:3000.
Sample(30 ug of whole cell lysate). A:Hep G2. B:MOLT4. 12% SDS PAGE. TA308687 diluted at 1:1000
Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Pyruvate Dehydrogenase E1 alpha antibody (TA308687) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha(TA308687) antibody at 1:200 dilution.
Western blot analysis of extracts from A549, HepG2 and Jurkat cells, using PDHA1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PDHA1 antibody.The picture on the right is treated with the synthesized peptide.
Predicted band size: 43 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using PDHA1 antibody.
Immunofluorescence analysis of U2OS cell using PDHA1 antibody. Blue: DAPI for nuclear staining.
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [TA336570] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretin
Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [TA336570] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubuli
Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293,1299,mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb(dilution 1:100).
WB Suggested Anti-PDHA1 Antibody; Positive Control: Lane 1: 60ug human NT2 cell line; Primary Antibody Dilution: 1: 500; Secondary Antibody: IRDye 800 CW goat anti-rabbit from Li-COR Bioscience; Secondry Antibody Dilution: 1: 20, 000; Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
WB Suggested Anti-PDHA1 Antibody; Positive Control: Lane 1: 80ug mouse brain extract; Primary Antibody Dilution: 1: 500; Secondary Antibody: IRDye 800 CW goat anti-rabbit from Li-COR Bioscience; Secondry Antibody Dilution: 1: 20, 000;
WB Suggested Anti-PDHA1 Antibody Titration: 5.0 ug/ml; Positive Control: HepG2 cell lysate.PDHA1 is supported by BioGPS gene expression data to be expressed in HepG2
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308687 | PDHA1 antibody |
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| Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA502696 | PDHA1 antibody |
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| PDHA1 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10) | |||||||
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Mouse | IgG2b | OTI2C10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA502704 | PDHA1 antibody |
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| PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
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Mouse | IgG2b | OTI2B10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA311767 | PDHA1 antibody |
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| Rabbit polyclonal anti-PDHA1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321101 | PDHA1 antibody |
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| Anti-PDHA1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327210 | PDHA1 antibody |
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| Rabbit anti-PDHA1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336570 | PDHA1 antibody |
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| Rabbit Polyclonal Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody | |||||||
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [TA336570] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretin](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-93479-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | ICC/IF, WB |
50 µl /
€590.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347015 | PDHA1 antibody |
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| Mouse Monoclonal pyruvate dehydrogenase (lipoamide) alpha 1 Antibody | |||||||
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Mouse | IgG1 | 3H2-F8-B5 | Purified | ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP53240PU-N | PDHA1 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP53241PU-N | PDHA1 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201831 | PDHA1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201831BN | PDHA1 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201831LP | PDHA1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201831RB | PDHA1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301831 | PDHA1 |
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| Recombinant protein of human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY432934 | PDHA1 overexpression lysate |
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0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. | ||
| LY432995 | PDHA1 overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY433066 | PDHA1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |