PDK1 antibody
Principal name
PDK1 antibody
Alternative names for PDK1 antibody
Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial
Gene ID
5163 (PDK1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of PDK1 antibody
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712):1098-101.
Detection limit for recombinant GST tagged PDK1 is approximately 30ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK1 is approximately 3ng/ml as a capture antibody.
The PDK1 polyclonal antibody ( Cat # PAB4561 ) is used in Western blot to detect PDK1 in mouse kidney tissue lysate .
PDK1 polyclonal antibody ( Cat # PAB6898 ) ( 1 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
PDK1, 29-436 aa, His-tagged: 15% SDS-PAGE (3 µg)
The anti-PDK1 Pab is used in Western blot to detect PDK1 in mouse kidney tissue lysate.
Western blot analysis of PDK1 in human heart lysate with PDK1 polyclonal antibody at 2 µg/ml ( Cat# PAB12077 ).
Western blot analysis of PDK1 in human heart lysate with PDK1 polyclonal antibody at 0.5 µg/ml ( Cat# PAB12078 ).
ICC/IF analysis of PDK1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human PDK1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green).
Flow cytometry analysis of PDK1 in Balb/3T3 cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Western blot analysis: The extracts of rat heart (60 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Hum. Adult Placenta; Host: Rabbit. Target Name: CHAD . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; PDK1 antibody - middle region (ARP31776_P050) in Hum. Adult Placenta cells using Western Blot
Human Jurkat; Host:Rabbit. Target Name:PDK2. Sample Tissue:Human Jurkat. Antibody Dilution: 1.0ug/ml. PDK1 is supported by BioGPS gene expression data to be expressed in Jurkat.; PDK1 antibody - middle region (ARP31776_P050) in Human Jurkat cells using Western Blot
Human MCF-7; WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; PDK1 antibody - middle region (ARP31776_P050) in Human MCF-7 cells using Western Blot
Skeletal muscle. Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Analysis of PDK1: Lane 1: MWMLane 2: HeLaLane 3: Rat BrainLane 4: Mouse BrainLane 5: VeroLane 6: ESK-4Lane 7: RK-13Lane 8: MDCK
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 antibody Cat.-No AM06566SU-N with DAB staining.
Immunofluorescence analysis of HELA cells using PDK1 antibody Cat.-No AM06566SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using PDK1 antibody Cat.-No AM06566SU-N against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
Flow Cytometric analysis of Lovo cells using PDK1 antibody Cat.-No AM06566SU-N (green) and negative control (purple).
Western blot analysis of PDK1 expression in LNCaP (A); A431 (B) whole cell lysates.
Detection of PDK1 in human heart lysate at 2 ug/ml
Western Blot: Analysis of HeLa lysates using PDK1 antibody
Immunocytochemistry/Immunofluorescence: PDK1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Detection of PDK1 in human heart lysate at 0.5 ug/ml.
TA303069 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from Hela cells, using PDK1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate
2Hum. Adult Placenta; Host: Rabbit; Target Name: CHAD; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0ug/ml
Host:Rabbit; Target Name:PDK2; Sample Tissue:Human Jurkat; Antibody Dilution: 1.0ug/mlPDK1 is supported by BioGPS gene expression data to be expressed in Jurkat
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06566SU-N | PDK1 antibody |
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Mouse | IgG1 | 4A11 | Ascites | Hu, Mky, Rt | E, F, ICC/IF, P, WB |
0.1 ml /
€410.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA301454 | PDK1 antibody |
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| Rabbit Polyclonal Antibody against PDK1 | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA301455 | PDK1 antibody |
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| Rabbit Polyclonal Antibody against PDK1 | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
| TA303069 | PDK1 antibody |
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| Goat Polyclonal Antibody against PDK1 | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA326285 | PDK1 antibody |
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| Rabbit polyclonal PDK1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AM09090PU-N | PDK1 (29-436) antibody |
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Mouse | IgG1 | AT2D6 | Purified | Hu, Rt | E, F, ICC/IF, WB |
0.1 ml /
€330.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP13579PU-N | PDK1 (N-term) antibody |
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Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| AP22886PU-N | PDK1 antibody |
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Rabbit | Purified | Can, Hu, Mky, Ms, Por, Rb, Rt | P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305127 | PDK1 |
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| Recombinant protein of human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||