PDK4 antibody
Principal name
PDK4 antibody
Alternative names for PDK4 antibody
Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial
Ncbi ID
9606, 10090, NP_002603, NP_002603.1, NP_038771.1, NM_053551, NM_002612
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PDK4 antibody
PDK4 (pyruvate dehydrogese kise isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptiol initiation complex responsible for PDK4 activation. Insulin promotes ictivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogese complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.
General readings
Kimura,K., (2006) Genome Res. 16 (1), 55-65
Western blot analysis of PDK4 ( arrow ) using PDK4 polyclonal antibody ( Cat # PAB4564 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PDK4 gene ( Lane 2 ) ( Origene Technologies ) .
The PDK4 polyclonal antibody ( Cat # PAB4564 ) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate .
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody ( Cat # PAB4565 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
Western blot analysis of PDK4 polyclonal antibody ( Cat # PAB4565 ) in CEM cell line lysates ( 35 µg/lane ) . PDK4 ( arrow ) was detected using the purified PDK4 polyclonal antibody ( Cat # PAB4565 ) ( 1 : 60 dilution ) .
Western blot analysis of PDK4 polyclonal antibody ( Cat # PAB4566 ) in HL-60 cell line lysates ( 35 µg/lane ) . PDK4 ( arrow ) was detected using the purified PDK4 polyclonal antibody ( Cat # PAB4566 ) ( 1 : 60 dilution ) .
Detection limit for recombinant GST tagged PDK4 is approximately 0.1ng/ml as a capture antibody.
Human Muscle; PDK4 antibody - N-terminal region (ARP32787_P050) in Human Muscle cells using Immunohistochemistry
Human Huh7, HepG2; . Sample Type: Huh7 HepG2 (50ug). Primary Antibody Dilution: 1:500 . Image Submitted By: Partha Kasturi. University of Kansas Medical Center .; PDK4 antibody - N-terminal region (ARP32787_P050) in Human Huh7, HepG2 cells using Western Blot
Human 293T; WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; PDK4 antibody - N-terminal region (ARP32787_P050) in Human 293T cells using Western Blot
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PDK4 Antibody in CEM cell line lysates (35ug/lane). PDK4 (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-PDK4 Antibody (Center) in HL60 cell line lysates (35ug/lane).PDK4(arrow) was detected using the purified Pab (1:60dilution).
Western Blot analysis of PDK4 expression in transfected 293T cell line (H00005166-T03) by PDK4 MaxPab polyclonal antibody.Lane 1: PDK4 transfected lysate(46.50 KDa).Lane 2: Non-transfected lysate.
Human Adrenal (formalin-fixed, paraffin-embedded) stained with PDK4 antibody AP22786PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with PDK4 antibody AP22786PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
PDK4 Antibody (C396) western blot of K562 cell line lysates (35 ug/lane). The PDK4 antibody detected the PDK4 protein (arrow).
The PDK4 Antibody is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human Adrenal tissue reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were incubated with TA302046 PDK4 (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the PDK4 mainly to the cytoplasm.
The PDK4 Antibody (C-term) (Cat# TA302046) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
PDK4 Antibody (C396) (Cat. #TA302046) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PDK4 antibody detected the PDK4 protein (arrow).
Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PDK4 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T
Sample Type: Huh7 HepG2 (50ug); Primary Antibody Dilution: 1:500 ; Image Submitted By: Partha Kasturi; University of Kansas Medical Center
Human Muscle
Western Blot: PDK4 Antibody [TA336725] - Western Blot on human heart protein lysate.
Western Blot: PDK4 Antibody [TA336726] - Western Blot on human heart protein lysate.
Immunocytochemistry/Immunofluorescence: PDK4 Antibody [TA336726] - PDK4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302046 | PDK4 (C-term) antibody |
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| Rabbit Polyclonal Antibody against PDK4 (C-term) | |||||||
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Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AP22786PU-N | PDK4 (C-term) antibody |
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 |
Rabbit | Purified | Hu, Ms | ICC/IF, P, WB |
0.2 ml /
€425.00
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| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA321507 | PDK4 antibody |
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| Anti-PDK4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA321508 | PDK4 antibody |
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| Anti-PDK4 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336725 | PDK4 antibody |
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| Rabbit Polyclonal PDK4 Antibody | |||||||
![Western Blot: PDK4 Antibody [TA336725] - Western Blot on human heart protein lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-07047-1-W.jpg) |
Rabbit | Purified | Hu, Ms | WB |
0.1 ml /
€450.00
|
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| OriGene Technologies, Inc. | |||||||
| TA336726 | PDK4 antibody |
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| Rabbit Polyclonal PDK4 Antibody | |||||||
![Western Blot: PDK4 Antibody [TA336726] - Western Blot on human heart protein lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-07049-1-W.jpg) |
Rabbit | Purified | Hu | ICC/IF, WB |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP13583PU-N | PDK4 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP13584PU-N | PDK4 (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301656 | PDK4 |
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| Recombinant protein of human pyruvate dehydrogenase kinase, isozyme 4 (PDK4) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205803L2V | Lenti ORF particles, Pdk4 (GFP-tagged ORF) - Rat pyruvate dehydrogenase kinase, isozyme 4 (Pdk4), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR205803L1V | Lenti ORF particles, Pdk4 (Myc-DDK-tagged ORF) - Rat pyruvate dehydrogenase kinase, isozyme 4 (Pdk4), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||