PDLIM1 antibody
Principal name
PDLIM1 antibody
Alternative names for PDLIM1 antibody
CLIM1, CLP36, CLP-36, Elfin, PDZ and LIM domain protein 1, LIM domain protein CLP-36, C-terminal LIM domain protein 1
Ncbi ID
9606, NP_058557, NP_066272.1, NP_066272, NM_020992, NP_058557.2, NM_017365
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PDLIM1 antibody
- Kit Component:
- - KN313054G1, Pdlim1 gRNA vector 1 in pCas-Guide vector
- KN313054G2, Pdlim1 gRNA vector 2 in pCas-Guide vector
- KN313054D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Silverberg, M.S., et al. Eur. J. Hum. Genet. 15(3):328-335(2007)
Miehe, U., et al. Histochem. Cell Biol. 126(4):465-471(2006)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
PDLIM1 polyclonal antibody (A01), Lot # 060717JCS1 Western Blot analysis of PDLIM1 expression in Jurkat ( Cat # L017V1 ).
Mouse NIH-3T3; WB Suggested Anti-PDLIM1 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; PDLIM1 antibody - middle region (ARP37229_T100) in Mouse NIH-3T3 cells using Western Blot
PDLIM1 polyclonal antibody ( Cat # PAB6554 ) staining ( 0.1 µg/ml ) of human lung lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
PDLIM1 monoclonal antibody (M02), clone 2D4. Western Blot analysis of PDLIM1 expression in Jurkat ( Cat # L017V1 ).
PDLIM1 MaxPab polyclonal antibody. Western Blot analysis of PDLIM1 expression in human placenta.
Western Blot analysis of PDLIM1 expression in transfected 293T cell line (H00009124-T01) by PDLIM1 MaxPab polyclonal antibody.Lane 1: PDLIM1 transfected lysate(36.19 KDa).Lane 2: Non-transfected lysate.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human pancreas.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human liver.
Western Blot analysis of PDLIM1 expression in transfected 293T cell line (H00009124-T01) by PDLIM1 MaxPab polyclonal antibody.Lane 1: PDLIM1 transfected lysate(36.10 KDa).Lane 2: Non-transfected lysate.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in HepG2.
AP08805PU-N at 5 µg/ml, staining PDLIM1 in Placenta tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded).
Antibody (AP08805PU-N at 0.1 µg/ml) staining of Human lung lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
PDLIM1 MaxPab polyclonal antibody. Western Blot analysis of PDLIM1 expression in human placenta.
Western Blot analysis of PDLIM1 expression in transfected 293T cell line (H00009124-T01) by PDLIM1 MaxPab polyclonal antibody.Lane 1: PDLIM1 transfected lysate(36.19 KDa).Lane 2: Non-transfected lysate.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human pancreas.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in HepG2.
PDLIM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human liver.
Western Blot analysis of PDLIM1 expression in transfected 293T cell line (H00009124-T01) by PDLIM1 MaxPab polyclonal antibody.Lane 1: PDLIM1 transfected lysate(36.10 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PDLIM1 transfected lysate using anti-PDLIM1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PDLIM1 MaxPab mouse polyclonal antibody (B01) (H00009124-B01).
Human HeLa; WB Suggested Anti-PDLIM1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. PDLIM1 is supported by BioGPS gene expression data to be expressed in HeLa.; PDLIM1 antibody - C-terminal region (ARP61889_P050) in Human HeLa cells using Western Blot
Western blot analysis of PDLIM1 Antibody (C-term) Cat.-No AP53245PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the PDLIM1 antibody detected the PDLIM1 protein (arrow).
Immunohistochemical analysis of PDLIM1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PDLIM1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PDLIM1 expression in HeLa (A); Saos2 (B); NIH3T3 (C) whole cell lysates.
Immunoprecipitation of PDLIM1 from 0.5mg HepG2 whole cell extract lysate using 5ug of Anti-PDLIM1 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-PDLIM1 Antibody.
TA302914 staining (0.1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts from Jurkat cells, COLO205 cells, 293 cells, HepG2 cells and HUVEC cells, using PDLIM1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PDLIM1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using PDLIM1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-PDLIM1 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate
Western blot analysis on K562 and Jurkat cell lysate using CLIM1 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302914 | PDLIM1 (C-term) antibody |
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| Goat Polyclonal Antibody against PDLIM1 | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA312393 | PDLIM1 antibody |
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| Rabbit polyclonal anti-PDLIM1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA347513 | PDLIM1 antibody |
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| Rabbit Polyclonal Anti-CLIM1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP08805PU-N | PDLIM1 (316-329) antibody |
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 |
Goat | IgG | Aff - Purified | Elephant, Hu, Platypus | E, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP53245PU-N | PDLIM1 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301674 | PDLIM1 |
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| Recombinant protein of human PDZ and LIM domain 1 (PDLIM1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208740L2V | Lenti ORF particles, Pdlim1 (GFP-tagged ORF) - Rat PDZ and LIM domain 1 (Pdlim1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208740L1V | Lenti ORF particles, Pdlim1 (Myc-DDK-tagged ORF) - Rat PDZ and LIM domain 1 (Pdlim1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||