PDLIM5 antibody
Principal name
PDLIM5 antibody
Alternative names for PDLIM5 antibody
Enigma homolog, ENH, Enigma-like PDZ and LIM domains protein
Ncbi ID
NP_001011513, NP_006448, 9606, NP_001011513.2, NM_006457, NM_001011513, NM_053326
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of PDLIM5 antibody
PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.
General readings
Lasorella,A., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (13), 4976-4981
Human HepG2; WB Suggested Anti-PDLIM5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - middle region (ARP34273_T100) in Human HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-PDLIM5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - middle region (ARP34274_P050) in Human HepG2 cells using Western Blot
Hum. Adult Placenta; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Heart; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Hum. Fetal Heart cells using Western Blot
Human HepG2; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Human HepG2 cells using Western Blot
Hum. Fetal Liver; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Fetal Brain; Host: Rabbit . Target Name: PDLIM5 . Sample Tissue: Human Fetal Brain . Antibody Dilution: 1.0ug/ml.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Hum. Fetal Brain cells using Western Blot
Human HepG2; WB Suggested Anti-PDLIM5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in Human HepG2 cells using Western Blot
HepG2; Host: Rabbit. Target Name:PDLIM5. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5.0 ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 12%. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - N-terminal region (ARP38795_P050) in HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-PDLIM5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2.; PDLIM5 antibody - C-terminal region (ARP38796_T100) in Human HepG2 cells using Western Blot
PDLIM5 polyclonal antibody (A02), Lot # NNR0051021QC01. Western Blot analysis of PDLIM5 expression in NIH/3T3.
PDLIM5 polyclonal antibody (A02), Lot # NNR0051021QC01 Western Blot analysis of PDLIM5 expression in Hela NE ( Cat # L013V3 ).
Immunofluorescence of monoclonal antibody to PDLIM5 on A-431 cell. [antibody concentration 10 ug/ml]
PDLIM5 monoclonal antibody (M01), clone 3E11-F6 Western Blot analysis of PDLIM5 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged PDLIM5 is approximately 3ng/ml as a capture antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDLIM5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
HEK293T cells transfected with either RC200592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDLIM5 antibody(TA504448), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PDLIM5 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
HEK293T cells transfected with either RC200592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDLIM5 antibody(TA504449), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
Western blot analysis of extracts of various cell lines, using PDLIM5 antibody.
Immunofluorescence analysis of U20S cell using PDLIM5 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-PDLIM5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate; There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2
WB Suggested Anti-PDLIM5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate; There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2
WB Suggested Anti-PDLIM5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate; There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2
Host: Rabbit; Target Name: PDLIM5; Sample Tissue: Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: PDLIM5; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: PDLIM5; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-PDLIM5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332862 | PDLIM5 antibody |
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| Rabbit anti-PDLIM5 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA504445 | PDLIM5 antibody |
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| PDLIM5 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5) | |||||||
 |
Mouse | IgG2b | OTI1B5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504448 | PDLIM5 antibody |
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| PDLIM5 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) | |||||||
 |
Mouse | IgG2a | OTI1H10 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504449 | PDLIM5 antibody |
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| PDLIM5 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11) | |||||||
 |
Mouse | IgG2a | OTI1A11 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA504505 | PDLIM5 antibody |
||||||
| PDLIM5 mouse monoclonal antibody, clone OTI1C11 (formerly 1C11) | |||||||
 |
Mouse | IgG2b | OTI1C11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300592 | PDLIM5 (transcript variant 1) |
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| Recombinant protein of human PDZ and LIM domain 5 (PDLIM5), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209090L2V | Lenti ORF particles, Pdlim5 (GFP-tagged ORF) - Rat PDZ and LIM domain 5 (Pdlim5), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| RR209090L1V | Lenti ORF particles, Pdlim5 (Myc-DDK-tagged ORF) - Rat PDZ and LIM domain 5 (Pdlim5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||