PDPK1 antibody
Principal name
PDPK1 antibody
Alternative names for PDPK1 antibody
PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1
Ncbi ID
NP_002604.1, 9606, NP_002604, NM_002613, NM_031268, NP_112558, NP_035192.2, NM_011062
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C)
Background of PDPK1 antibody
- Kit Component:
- - KN313062G1, Pdpk1 gRNA vector 1 in pCas-Guide vector
- KN313062G2, Pdpk1 gRNA vector 2 in pCas-Guide vector
- KN313062D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lin,H.J., (2005) Br. J. Cancer 93 (12), 1372-1381
Western Blot analysis of PDK-1 expression with Jurkat cell lysate.
PDPK1 polyclonal antibody (A02), Lot # 051017JC01 Western Blot analysis of PDPK1 expression in MES-SA/Dx5 ( Cat # L021V1 ).
Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (Phospho-Ser241) Cat. AP02307PU
Figure 3. Immunofluorescence staining of methanol-fixed Hela cells using PDK1 antibody (Phospho-Ser241) Cat. AP02307PU
Figure 2. Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 antibody (phospho-Ser241) Cat. AP02307PU
Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (AP02549PU-S; AP02549PU-N).
Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1 antibody
Figure 2. Western blot analysis of extracts from MDA-MB-435 cells using PDK1 antibody (AP02549PU-S; AP02549PU-N).
Western Blot analysis of extracts from MCF, 293 and PC12 cells using PDK1 antibody
Western Blot analysis of PDPK1 expression in transfected 293T cell line (H00005170-T01) by PDPK1 MaxPab polyclonal antibody.Lane 1: PDPK1 transfected lysate(47.19 KDa).Lane 2: Non-transfected lysate.
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in K-562.
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in NIH/3T3.
Figure 1. Staining PDPK1 in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Human Jurkat; WB Suggested Anti-PDPK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; PDPK1 antibody - middle region (ARP30313_P050) in Human Jurkat cells using Western Blot
Dot blot analysis of anti-PDPK1-pSer396 Phospho-specific Pab (CAt#AP12874PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
AP15725PU PDPK1 antibody staining of Human Breast Carcinoma.
Western blot using PDPK1 polyclonal antibody ( Cat # PAB10309 ) shows detection of myc-tagged human PDPK1 at 60 kDa in ~10 µg of a virus infected Sf9 cell lysate ( arrow ).The nitrocellulose membrane was probed overnight at 4°C with the primary antibody diluted 1 : 750 in 1% non-fat dry milk.HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended.
Western blot of A-431 cells untreated (lane 1) or treated with pervanadate (lane 2). Blots were probed with PDPK1 polyclonal antibody ( Cat # PAB7885 ) .
Western blot of A-431 cells untreated (lanes 1) or treated with pervanadate (lanes 2, 3 & 4). Blots were probed with PDPK1 (phospho Y9) polyclonal antibody ( Cat # PAB7926 ). The latter was used in the presence of no peptide (lane 2), phospho-PDK1 (Tyr-9) peptide (lane 3), or an unrelated phosphotyrosine peptide (lane 4).
Dot blot analysis of PDPK1 (phospho S396) polyclonal antibody ( Cat # PAB8131 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Western blot analysis of PDPK1 expression in Jurkat cell lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RPS6KA3 and PDPK1 HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of PDPK1 expression in transfected 293T cell line (H00005170-T01) by PDPK1 MaxPab polyclonal antibody.Lane 1: PDPK1 transfected lysate(47.19 KDa).Lane 2: Non-transfected lysate.
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in K-562.
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in NIH/3T3.
Immunoprecipitation of PDPK1 transfected lysate using anti-PDPK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PDPK1 MaxPab mouse polyclonal antibody (B01) (H00005170-B01).
Human Testis, Spermatogonia: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of HeLa cell lysates with PDK1 antibody at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody AP55743PU-N (left)or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PDPK1 (pS241) expression in HeLa (A); MCF7 (B); NIH3T3 (C); PC12 EGF-treated (D) whole cell lysates.
ELISA: PDPK1 Antibody
Western blot using affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 µg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4° C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Predicted band size: 63 kDa . Positive control: MCF, 293 and PC12 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: MCF, 293 and PC12 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PDK1 (Ab-241) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using PDPK1 antibody at dilution 1/100. (Original magnification: ×200)
Western blot analysis of PDK1 expression in EGF treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using PDPK1 antibody.
Immunofluorescence analysis of U2OS cell using PDPK1 antibody. Blue: DAPI for nuclear staining.
Human Breast Carcinoma stained with anti-PDK-1 antibody.
WB Suggested Anti-PDPK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312024 | PDPK1 antibody |
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| Rabbit polyclonal PDK1 (Tyr9) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA590136 | PDPK1 antibody |
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| Rabbit Polyclonal PDPK1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP31740PU-N | PDPK1 antibody |
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 |
Rabbit | IgG | Purified | Hu | E, P, WB |
50 µl /
€425.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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