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PEA15 antibody

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Principal name

PEA15 antibody

Alternative names for PEA15 antibody

Astrocytic phosphoprotein PEA-15, PED, Phosphoprotein enriched in diabetes

SwissProt ID

Q15121 (Human), Q5U318 (Rat), Q62048 (Mouse)

Gene ID

8682 (PEA15), 364052 (Pea15a), 18611

Ncbi ID

NP_003759.1, 9606, NP_003759, NP_001013249.1, NP_035193.1, NM_001013231, NM_003768

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of PEA15 antibody

PEA-15 (phosphoprotein enriched in astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 co-localizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, phosphoprotein enriched in diabetes, for its elevated expression in type 2 diabetic patients.

General readings

Beguinot F., et al. (2009) Am J Physiol Endocrinol Metab. 297(3):E592-601.
Ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.

AP06559PU-N - PEA15
Immunohistochemistry (IHC) analyzes of PEA-15 antibody (Cat.-No.: AP06559PU-N) in paraffin-embedded human colon carcinoma tissue.
AP06559PU-N - PEA15
Western blot(WB) analysis of PEA-15 antibody (Cat.-No.: AP06559PU-N) in extracts from Jurkat cells treated with PMA 125ng/ml 30'.
H00008682-B01 - PEA15
Western Blot analysis of PEA15 expression in transfected 293T cell line (H00008682-T01) by PEA15 MaxPab polyclonal antibody.Lane 1: PEA15 transfected lysate(11.00 KDa).Lane 2: Non-transfected lysate.
ARP48232_P050 - PEA15
Transfected MCF7; WB Suggested Anti-PEA15 Antibody . . Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Yann Wallez (Sanford-Burnham Medical Research Institute); PEA15 antibody - middle region (ARP48232_P050) in Transfected MCF7 cells using Western Blot
ARP48232_P050 - PEA15
Human 721_B; WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; PEA15 antibody - middle region (ARP48232_P050) in Human 721_B cells using Western Blot
AR09282PU-L - PEA15
AR09282PU-L - PEA15
Recombinant human PEA-15, 1-130 aa: 15% SDS-PAGE (3 µg)
AP18148PU-N - PEA15
(LEFT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in T47D cell line lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in mouse lung tissue lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab.
A0545-1 - PEA15
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using PEA-15 (Phospho-Ser116) Antibody. The picture on the right is treated with the synthesized peptide.
A0545-1 - PEA15
Western blot analysis of extracts from COS7 cells treated with INSULIN 0.01U/ML 15', using PEA-15 (Phospho-Ser116) Antibody. The lane on the right is treated with the synthesized peptide.
A0711-1 - PEA15
Immunohistochemistry analysis of paraffin-embedded human heart, using PEA-15 (Phospho-Ser104) Antibody. The picture on the right is treated with the synthesized peptide.
A0711-1 - PEA15
Western blot analysis of extracts from COS7 cells treated with TNF 20ng/ml 5', using PEA-15 (Phospho-Ser104) Antibody. The lane on the right is treated with the synthesized peptide.
B0545-1 - PEA15
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PEA-15 (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide.
B0545-1 - PEA15
Immunofluorescence analysis of HeLa cells, using PEA-15 (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide.
B0545-1 - PEA15
Western blot analysis of extracts from Jurkat cells, treated with PMA 125ng/ml 30', using PEA-15 (Ab-116) Antibody. The lane on the right is treated with the synthesized peptide.
B0711-1 - PEA15
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PEA-15 (Ab-104) Antibody. The picture on the right is treated with the synthesized peptide.
C13100-1 - PEA15
Immunofluorescence analysis of MCF7 cells, using PEA15 Antibody. The picture on the right is treated with the synthesized peptide.
ARP58923_P050 - PEA15
Human Brain; WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; PEA15 antibody - C-terminal region (ARP58923_P050) in Human Brain cells using Western Blot
ARP58923_P050 - PEA15
Transfected MCF7; WB Suggested Anti-PEA15 Antibody . . Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Yann Wallez (Sanford-Burnham Medical Research Institute); PEA15 antibody - C-terminal region (ARP58923_P050) in Transfected MCF7 cells using Western Blot
AP55709PU-S - PEA15
Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody AP55709PU-N (left)or the same antibody preincubated with blocking peptide (right).
AP55709PU-S - PEA15
Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody AP55709PU-N.The lane on the right is treated with the antigen-specific peptide.
TP305507 - PEA15
TA314790 - PEA15
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 antibody.The picture on the right is treated with the synthesized peptide.
TA346580 - PEA15
WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate
TA346580 - PEA15
WB Suggested Anti-PEA15 Antibody; Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Goat anti-rabbit-HRP; Secondry Antibody Dilution: 1: 5000;
TA314791 - PEA15
Western blot analysis of extracts from COS cells, treated with TNF (20ng/ml, 5mins), using PEA-15 (Phospho-Ser104) antibody.The lane on the right is treated with the synthesized peptide.
TA314791 - PEA15
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody.The picture on the right is treated with the synthesized peptide.

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PEA15 antibody
  Rabbit polyclonal anti-PEA-15 antibody  
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

PEA15 antibody
  Rabbit polyclonal PEA-15 (Ser104) antibody(Phospho-specific)  
Western blot analysis of extracts from COS cells, treated with TNF (20ng/ml, 5mins), using PEA-15 (Phospho-Ser104) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

PEA15 antibody
Immunohistochemistry (IHC) analyzes of PEA-15 antibody (Cat.-No.: AP06559PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

PEA15 (C-term) antibody
(LEFT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in T47D cell line lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in mouse lung tissue lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

PEA15 pSer104 antibody
Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody AP55709PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €300.00
  OriGene Technologies GmbH
+1 additional image

PEA15 pSer104 antibody
Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody AP55709PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €230.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PEA15
  Recombinant protein of human phosphoprotein enriched in astrocytes 15 (PEA15)  
PEA15 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pea15 (GFP-tagged ORF) - Rat phosphoprotein enriched in astrocytes 15A (Pea15a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pea15 (GFP-tagged ORF) - Rat phosphoprotein enriched in astrocytes 15A (Pea15a), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pea15 (Myc-DDK-tagged ORF) - Rat phosphoprotein enriched in astrocytes 15A (Pea15a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pea15 (Myc-DDK-tagged ORF) - Rat phosphoprotein enriched in astrocytes 15A (Pea15a), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (6)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (6)
Target
  1. PEA15 (7)
Property
  1. C-term (1)
  2. pSer104 (2)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
Applications
  1. Paraffin Sections (P) (5)
  2. Western blot / Immunoblot (WB) (5)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (3)
  2. Purified (2)
Host
  1. Rabbit (6)
Images
  1. 1 image (7)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
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