PECI antibody
Principal name
PECI antibody
Alternative names for PECI antibody
DRS1, DRS-1, HCA88, NY-REN-1, NYREN1, Peroxisomal 3,2-trans-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, Hepatocellular carcinoma-associated antigen 88, Renal carcinoma antigen NY-REN-1
Ncbi ID
NP_006108, 9606, NP_001159482.1, NP_001159482, NP_001103801.1, NM_001110332, NM_001110331, NM_011868, NM_206836, NM_001166010, NM_006117
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of PECI antibody
- Kit Component:
- - KN304982G1, Eci2 gRNA vector 1 in pCas-Guide vector
- KN304982G2, Eci2 gRNA vector 2 in pCas-Guide vector
- KN304982D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dongyan Zhang,et al. (2002) J. Biol. Chem. 277: 9127-9132.
Immunofluorescence of purified MaxPab antibody to PECI on HeLa cell. [antibody concentration 10 ug/ml]
PECI MaxPab polyclonal antibody. Western Blot analysis of PECI expression in human liver.
Western Blot analysis of PECI expression in transfected 293T cell line (H00010455-T01) by PECI MaxPab polyclonal antibody.Lane 1: PECI transfected lysate(40.04 KDa).Lane 2: Non-transfected lysate.
PECI MaxPab polyclonal antibody. Western Blot analysis of PECI expression in Jurkat.
Human HepG2; WB Suggested Anti-PECI Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. ECI2 is supported by BioGPS gene expression data to be expressed in HepG2.; PECI antibody - middle region (ARP48692_P050) in Human HepG2 cells using Western Blot
Western blot analysis of PECI Antibody (C-term) (AP18149PU-N) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.
Recombinant human PECI, 1-364 aa, His-tagged
Immunhistochemistry analysis in human skeletal muscle (Formalin-fixed, Paraffin-embedded) using ECI2 / PECI antibody Cat.-No. AP32316PU-N.
Western blot analysis of extracts from 293 cells, using ECI2 / PECI antibody Cat.-No. AP32316PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of PECI staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PECI expression in Jurkat (A); Raji (B); A431 (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECI (RC204994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECI.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504275)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504275)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504275)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504275)
Anti-PECI mouse monoclonal antibody (TA504275) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECI(RC204994).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECI monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECI (RC204994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECI.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECI (RC204994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECI.
Western blot analysis of extracts from 293 cells, using PECI antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PECI antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-PECI Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate.ECI2 is supported by BioGPS gene expression data to be expressed in HepG2
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504275 | PECI antibody |
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| PECI mouse monoclonal antibody, clone OTI1C8 (formerly 1C8) | |||||||
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Mouse | IgG2b | OTI1C8 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
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| TA312299 | PECI antibody |
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| Rabbit polyclonal anti-PECI antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA504214 | PECI antibody |
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| PECI mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) | |||||||
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Mouse | IgG1 | OTI1H4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA504437 | PECI antibody |
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| PECI mouse monoclonal antibody, clone OTI2H10 (formerly 2H10) | |||||||
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Mouse | IgG1 | OTI2H10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP18149PU-N | PECI (C-term) antibody |
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Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP32316PU-N | PECI antibody |
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Rabbit | IgG | Aff - Purified | Hu | E, P, WB |
50 µl /
€425.00
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|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304994 | PECI (transcript variant 1) |
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| Recombinant protein of human peroxisomal D3,D2-enoyl-CoA isomerase (PECI), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328846 | PECI (transcript variant 3) |
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| Recombinant protein of human enoyl-CoA delta isomerase 2 (ECI2), transcript variant 3. | |||||
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Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC229188L2V | Lenti ORF particles, ECI2 (mGFP-tagged)-Human enoyl-CoA delta isomerase 2 (ECI2), transcript variant 2, 200 uL, >10^7 TU/mL |
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 |
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC229188L1V | Lenti ORF particles, ECI2 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 2 (ECI2), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||