Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » Pepsin antibody

Pepsin antibody

(reset)

Principal name

Pepsin antibody

Alternative names for Pepsin antibody

Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5

SwissProt ID

P00790 (Human), P00791 (Pig), P00792 (Bovin), P00793 (Chick), Q9GMY6 (Canfa)

Gene ID

643834 (PGA3), 396892 (PGA), 5222 (PGA5), 643847 (PGA4)

Ncbi ID

NP_001073276, NP_999038, NP_001073276.1, AAI52845.1, 9823, NM_014224

Available reactivities

Hu (Human), Por (Porcine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Pepsin antibody

PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor,pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.PGA5 is inhibited by ovUS-1, a uterine serpin.

General readings

Plebani, M., et al. Helicobacter 2(4):172-175(1997)
Fujinaga, M., et al. Protein Sci. 4(5):960-972(1995)
Zwiers, A., et al. Clin. Nephrol. 41(3):153-158(1994)
Ichinose, M., et al. Jpn. J. Cancer Res. 82(6):686-692(1991)
Athauda, S.B., et al. J. Biochem. 106(5):920-927(1989)

AP20789PU-N - Pepsin
Immunohistochemistry analysis of Pepsin A antibody Cat.-No AP20789PU-N in paraffin-embedded human lung carcinoma tissue.
AP20789PU-N - Pepsin
Western blot analysis of Pepsin A antiboy Cat.-No AP20789PU-N in extracts from K562 cells.
AP53266PU-N - Pepsin
Western blot analysis of Pepsin Antibody (N-term) Cat.-No AP53266PU-N  in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the PGA3 antibody detected the PGA3 protein (arrow).
AP53267PU-N - Pepsin
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with PGA4 Antibody (C-term) Cat.-No AP53267PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP53267PU-N - Pepsin
Western blot analysis of PGA4 Antibody (C-term) Cat.-No AP53267PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the PGA4 antibody detected the PGA4 protein (arrow).
AP53268PU-N - Pepsin
Western blot analysis of Pepsin Antibody (C-term) Cat.-No AP53268PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the PGA5 antibody detected the PGA5 protein (arrow).
H00005222-D01P - Pepsin
PGA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of PGA5 expression in human liver.
H00005222-D01P - Pepsin
Western Blot analysis of PGA5 expression in transfected 293T cell line (H00005222-T02) by PGA5 MaxPab polyclonal antibody.Lane 1: PGA5 transfected lysate(42.00 KDa).Lane 2: Non-transfected lysate.
H00005222-M02 - Pepsin
Immunofluorescence of monoclonal antibody to PGA5 on HeLa cell . [antibody concentration 10 ug/ml]
H00005222-M02 - Pepsin
PGA5 monoclonal antibody (M02), clone 4G9 Western Blot analysis of PGA5 expression in HepG2 ( Cat # L019V1 ).
AM06373SU-N - Pepsin
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining.
AM06373SU-N - Pepsin
Western blot analysis using PGA5 mouse mAb against HepG2 (Lane 1) and SMMC-7721 (Lane 2) cell lysate.
R1106PS - Pepsin
Immunohistochemistry of goat anti-pepsin antibody 1:100
R1106PS - Pepsin
Western blot of goat anti-Pepsin antibody Cat.-No. R1106P. Lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Pepsin primary antibody at 1/1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 35 kDa/35 kDa for Pepsin. Other band(s): None.
CPA1880-100ul - Pepsin A
Immunohistochemical analysis of Pepsin A staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1880-100ul - Pepsin A
Western blot analysis of Pepsin A expression in HepG2 (A) whole cell lysates.
AB0197-200 - Pepsin
TA322389 - Pepsin
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
TA322389 - Pepsin
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using PGA5 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA322390 - Pepsin
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA322390 - Pepsin
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PGA5 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323324 - Pepsin
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
TA323324 - Pepsin
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using PGA4 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323325 - Pepsin
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA323325 - Pepsin
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PGA4 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA338325 - Pepsin
WB Suggested Anti-PGA4 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Liver
  1. Quick-Filters:
  2. Cited (1)

12 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pepsin antibody
Immunohistochemistry analysis of Pepsin A antibody Cat.-No AP20789PU-N in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Published recently!

Pepsin antibody
Pepsin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €300.00
  SICGEN
 

OriGene recommends this product for Western blotting.

Pepsin antibody
  Anti-PGA4 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Pepsin antibody
  Anti-PGA4 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Pepsin antibody
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining. Mouse IgG1 2C1 Ascites Hu E, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

Pepsin (N-term) antibody
Western blot analysis of Pepsin Antibody (N-term) Cat.-No AP53266PU-N  in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the PGA3 antibody detected the PGA3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Pepsin (C-term) antibody
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with PGA4 Antibody (C-term) Cat.-No AP53267PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Pepsin (C-term) antibody
Western blot analysis of Pepsin Antibody (C-term) Cat.-No AP53268PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the PGA5 antibody detected the PGA5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Pepsin antibody
Pepsin Goat Serum Por C, E, IP, P, WB
2 ml / €1,345.00
  OriGene Technologies GmbH

Pepsin antibody
Pepsin Goat IgG Biotin Por C, E, P, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

Pepsin antibody
Pepsin Goat IgG HRP Por C, E, P, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

Pepsin antibody
Immunohistochemistry of goat anti-pepsin antibody 1:100 Goat IgG Purified Por C, E, IP, P, WB
0.1 mg / €515.00
  OriGene Technologies GmbH
+1 additional image

12 Item(s)

per page
12 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (12)
Clonality
  1. Polyclonal (11)
  2. Monoclonal (1)
Target
  1. Pepsin (12)
Property
  1. C-term (2)
  2. N-term (1)
Reactivity
  1. Human (Hu) (8)
  2. Porcine (Por) (4)
  3. Mouse (Ms) (3)
  4. Rat (Rt) (3)
  5. Canine (Can) (1)
  6. Monkey (Mky) (1)
Applications
  1. Western blot / Immunoblot (WB) (12)
  2. Paraffin Sections (P) (9)
  3. Enzyme Immunoassay (E) (7)
  4. Frozen sections (C) (4)
  5. Immunoprecipitation (IP) (2)
Presentation
  1. Aff - Purified (5)
  2. Purified (3)
  3. Ascites (1)
  4. Biotin (1)
  5. HRP (1)
  6. Serum (1)
Host
  1. Rabbit (6)
  2. Goat (5)
  3. Mouse (1)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (3)
  3. IHC (2)
  4. Frozen sections (C) (1)
Manufacturer
  1. OriGene Technologies (11)
  2. SICGEN (1)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn