Periostin antibody
Principal name
Periostin antibody
Alternative names for Periostin antibody
POSTN, OSF2, OSF-2, PN, Osteoblast-specific factor 2
Ncbi ID
NP_001129406, NP_001129406.1, 9606, NP_006466, NP_001185694.1, NM_001135935, NP_001129407, NM_001135934, BC031449, NM_006475, NM_001135936
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)
Background of Periostin antibody
Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
General readings
Zhu, M., et al. Gynecol. Oncol. 119(2):337-344(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Takada, M., et al. Biochem. Biophys. Res. Commun. 399(2):221-226(2010)
Sidhu, S.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14170-14175(2010)
Tischler, V., et al. BMC Cancer 10, 273 (2010).
12% SDS-PAGE separation of Human OSF-2: 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. Reduced and heated sample, 5µg/lane 3. Non-reduced and non-heated sample, 5µg/lane.
Human Heart; Anti-POSTN / Periostin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; POSTN antibody - middle region (ARP41571_P050) in Human Heart cells using Immunohistochemistry
Human Uterus; Anti-POSTN / Periostin antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; POSTN antibody - middle region (ARP41571_P050) in Human Uterus cells using Immunohistochemistry
Human Uterus; Rabbit Anti-POSTN Antibody. Catalog Number: arp41571. Paraffin Embedded Tissue: Human Uterus. Antibody Concentration: 5 ug/ml; POSTN antibody - middle region (ARP41571_P050) in Human Uterus cells using Immunohistochemistry
Human 293T; WB Suggested Anti-POSTN Antibody . Titration: 1 ug/ml. Positive Control: 293T cells lysate; POSTN antibody - middle region (ARP41571_P050) in Human 293T cells using Western Blot
721_B; WB Suggested Anti-POSTN Antibody Titration: 1 ug/ml. Positive Control: 721_B cell lysate; POSTN antibody - N-terminal region (ARP41570_P050) in 721_B cells using Western Blot
Formalin fixed and paraffin embedded human uterus tissue reacted with Periostin Antibody (C-term) Cat.-No AP53392PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Periostin Antibody (C-term) Cat.-No AP53392PU-N with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Periostin Antibody (C-term) Cat.-No AP53392PU-N in MCF-7, A375 cell line lysates (35ug/lane). This demonstrates the POSTN antibody detected the POSTN protein (arrow).
Representative Standard Curve
Representative Standard Curve
Anti-POSTN mouse monoclonal antibody (TA500070) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN(RC215156).
Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human periostin gene expression vector (Cat# RC215156)
POSTN Luminex with 2D10 Capture (TA600528) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human POSTN (TP327934)
POSTN Luminex with Anti-Tag 1B12 Capture (TA600050) and 2D10 Detection (TA700528) Antibodies. Substrate used: Recombinant Human POSTN (TP327934)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803712)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804575)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804575)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN.
Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.
Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein triplet.
Anti-POSTN (2ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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- Cited (2)
- Highly Validated (1)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500070 | Periostin antibody |
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| Periostin (Periostin) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1) | |||||||
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Mouse | IgM | OTI1A1 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
Top Selling product!
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| DP2019 | Periostin antibody |
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Rabbit | Azide Free | Chk, Hu, Ms, Rt | C, E, P, WB |
50 µg /
€420.00
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| OriGene Technologies GmbH | |||||||
Top Selling product! |
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| TA309211 | Periostin (C-term) antibody |
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| Rabbit Anti-Periostin C-terminal Antibody | |||||||
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Rabbit | IgG | Azide Free | Chk, Hu, Mam, Ms, Rt | P, WB |
0.1 ml /
€405.00
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| OriGene Technologies, Inc. | |||||||
Published recently! |
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| TA804575 | Periostin antibody |
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| POSTN mouse monoclonal antibody, clone OTI2B2 (formerly 2B2) | |||||||
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Mouse | IgG2a | OTI2B2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
Published recently!
|
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| AP53392PU-N | Periostin (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA309212 | Periostin (pan) antibody |
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| Rabbit Anti-Periostin pan Antibody | |||||||
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Rabbit | IgG | Azide Free | Amph, Chk, Fi, Hu, Mam, Ms, Rt | C, P, WB |
0.1 ml /
€405.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA320204 | Periostin (C-term) antibody |
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| Goat Polyclonal Periostin (POSTN) Antibody | |||||||
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA600528 | Periostin antibody |
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| Luminex validated purified POSTN mouse monoclonal antibody (clone 2D1, clone OTI2D10 (formerly 2D10) | |||||||
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Mouse | IgG1 | OTI2D10 | Purified | Hu | LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| TA700528 | Periostin antibody |
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| Luminex validated purified biotinylated POSTN mouse monoclonal antibody (clone 2D10), clone OTI2D10 (formerly 2D10) | |||||||
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Mouse | IgG1 | OTI2D10 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| TA803701 | Periostin antibody |
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| POSTN mouse monoclonal antibody, clone OTI6A9 (formerly 6A9) | |||||||
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Mouse | IgG1 | OTI6A9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA803712 | Periostin antibody |
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| POSTN mouse monoclonal antibody, clone OTI2F7 (formerly 2F7) | |||||||
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Mouse | IgG1 | OTI2F7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA803713 | Periostin antibody |
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| Purified POSTN mouse monoclonal antibody, clone OTI2D10 (formerly 2D10) | |||||||
 |
Mouse | IgG1 | OTI2D10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN215156 | POSTN - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN215156BN | POSTN - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN215156LP | POSTN - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN215156RB | POSTN - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP327281 | Periostin (transcript variant 2) |
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| Purified recombinant protein of Homo sapiens periostin, osteoblast specific factor (POSTN), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP327934 | Periostin (transcript variant 3) |
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| Recombinant protein of human periostin, osteoblast specific factor (POSTN), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| PA514X | Periostin (His-tagged Fusion Protein) |
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Human | Purified | > 90 % pure by SDS-PAGE. | E. coli |
0.1 mg /
€490.00
|
| OriGene Technologies GmbH | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100631 | Human Periostin/OSF2 ELISA Kit |
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1 kit /
€535.00
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|
| OriGene Technologies, Inc. |