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PFKFB1 antibody

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Principal name

PFKFB1 antibody

Alternative names for PFKFB1 antibody

F6PK, PFRX, PFK/FBPase 1, PFK-2 liver, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1

SwissProt ID

P07953 (Rat), P16118 (Human), P49872 (Bovin), P70266 (Mouse)

Gene ID

5207 (PFKFB1), 18639 (Pfkfb1)

Ncbi ID

9606, NP_002616, 5874, NP_002616.2, NM_002625, NP_032850.1, NM_008824

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of PFKFB1 antibody

PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) is a bifunctional enzyme, having both kinase and phosphatase activities residing on the same enzyme subunit but having distinct active sites. PFKFB regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis, phosphofructokinase. To date, four PFKFB isozymes (PFKFB 1-4) have been described, which show differences in their tissue distribution and kinetic properties in response to allosteric effectors and hormonal signals. Among the PFKFB's PFKFB3 has the highest kinase:phosphatase ratio, in part because it lacks the characteristic serine phosphorylation site near the N-terminal that down-modulates kinase activity. PFKFB3 was first described in the rapidly growing placenta. The glucolitic rate in placenta is accelerated by anoxia and by maternal diabetes. Cancer cells maintain a high glycolytic rate even in the presence of oxygen, a phenomenon known as the Warburg effect. The glycolytic rate in the placenta, another fast-growing tissue, is accelerated by anoxia and by maternal diabetes.

AP06742PU-N - PFKFB1/4
Western blot (WB) analysis of PFKFB1/4 antibody (Cat.-No.: AP06742PU-N) in extracts from HeLa cells.
PAB4020 - PFKFB1
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 polyclonal antibody ( Cat # PAB4020 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB4020 - PFKFB1
Western blot analysis of PFKFB1 polyclonal antibody ( Cat # PAB4020 ) in Jurkat cell lysate ( 35 µg/lane ) . PFKFB1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 240 dilution ) .
PAB4021 - PFKFB1
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 polyclonal antibody ( Cat # PAB4021 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB4021 - PFKFB1
Western blot analysis of PFKFB1 polyclonal antibody ( Cat # PAB4021 ) in Jurkat cell lysate ( 35 µg/lane ) . PFKFB1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 240 dilution ) .
H00005207-B01 - PFKFB1
Western Blot analysis of PFKFB1 expression in transfected 293T cell line (H00005207-T01) by PFKFB1 MaxPab polyclonal antibody.Lane 1: PFKFB1 transfected lysate(51.81 KDa).Lane 2: Non-transfected lysate.
AP15140PU-N - PFKFB1
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP15140PU-N - PFKFB1
Western blot analysis of anti-PFKFB1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PFKFB1(arrow) was detected using the purified Pab (1:240 dilution).
AP15141PU-N - PFKFB1
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining
AP15141PU-N - PFKFB1
Western blot analysis of anti-PFKFB1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). PFKFB1(arrow) was detected using the purified Pab (1:240 dilution).
AP20411PU-N - PFKFB1
Immunohistochemistry: AP20411PU-N PFK-2 liv antibody staining of Paraffin-Embedded Human liver carcinoma tissue.
AP20411PU-N - PFKFB1
Western blot (WB) analysis of AP20411PU-N PFK-2 liv antibody in extracts from HUVEC cells.
C11146-1 - PFKFB1/4
Western blot analysis of extracts from HeLa/HepG2/COLO205/293 cells, using PFKFB1/4 Antibody. The lane on the right is treated with the synthesized peptide.
CPA1875-100ul - PFKFB1/4
Western blot analysis of PFKFB1/4 expression in Jurkat (A); H1299 (B); HeLa (C) whole cell lysates.
TP324599 - PFKFB1
TP760672 - PFKFB1
TA314332 - PFKFB1
Western blot analysis of extracts from HUVEC cells and Jurkat cells, using PFKFB1 antibody.The lane on the right is treated with the synthesized peptide.
TA314333 - PFKFB1
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells, using PFKFB1/4 antibody.The lane on the right is treated with the synthesized peptide.

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PFKFB1 antibody
  Rabbit polyclonal anti-PFKFB1 antibody  
Western blot analysis of extracts from HUVEC cells and Jurkat cells, using PFKFB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

PFKFB1 antibody
  Rabbit polyclonal anti-PFKFB1/4 antibody  
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells, using PFKFB1/4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

PFKFB1 antibody
  Rabbit Polyclonal Anti-PFKFB1/4 Antibody  
PFKFB1 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

PFKFB1 antibody
  Rabbit Polyclonal Anti-PFKFB1 Antibody  
PFKFB1 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

PFKFB1 (N-term) antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

PFKFB1 (Center) antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

PFKFB1 (Liver) antibody
Immunohistochemistry: AP20411PU-N PFK-2 liv antibody staining of Paraffin-Embedded Human liver carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

PFKFB1/4 antibody
Western blot (WB) analysis of PFKFB1/4 antibody (Cat.-No.: AP06742PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PFKFB1
  Recombinant protein of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1)  
PFKFB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

PFKFB1 (full length, N-term HIS tag)
  Purified recombinant protein of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PFKFB1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PFKFB1 overexpression lysate
PFKFB1 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pfkfb1 (GFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pfkfb1 (GFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pfkfb1 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pfkfb1 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.
 

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (8)
  4. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (8)
Target
  1. PFKFB1 (9)
  2. PFKFB1/4 (1)
Property
  1. Center (1)
  2. Liver (1)
  3. N-term (1)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (6)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
Presentation
  1. Purified (6)
  2. Aff - Purified (2)
Host
  1. Rabbit (8)
Images
  1. 1 image (8)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Paraffin Sections (P) (3)
Species (Protein)
  1. Human (2)
Source
  1. E. coli (1)
  2. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (13)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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