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PFKFB3 antibody

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Principal name

PFKFB3 antibody

Alternative names for PFKFB3 antibody

iPFK-2, NY-REN-56, 6-phosphofructo-2-kinase, 6PF-2-K, fructose-2,6-biphosphatase 3, Fru-2,6-P2ASE brain/placenta-type isozyme, Renal carcinoma antigen NY-REN-56

SwissProt ID

O35552 (Rat), Q16875 (Human), Q28901 (Bovin)

Gene ID

5209 (PFKFB3), 117276 (Pfkfb3)

Ncbi ID

9606, NP_476476, NP_001138915.1, NM_001145443, NP_004557, NM_057135, NM_004566

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PFKFB3 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

H00005209-A01 - PFKFB3
PFKFB3 polyclonal antibody (A01), Lot # 060626JCS1 Western Blot analysis of PFKFB3 expression in SJCRH30 ( Cat # L027V1 ).
PAB4017 - PFKFB3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PFKFB3 polyclonal antibody ( Cat # PAB4017 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB4017 - PFKFB3
The PFKFB3 polyclonal antibody ( Cat # PAB4017 ) is used in Western blot to detect PFKFB3 in Jurkat cell lysate ( Lane 1 ) and mouse brain tissue lysate ( Lane 2 ) .
H00005209-M08 - PFKFB3
Western Blot analysis of PFKFB3 expression in transfected 293T cell line by PFKFB3 monoclonal antibody (M08), clone 3F3.Lane 1: PFKFB3 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.
H00005209-M08 - PFKFB3
Detection limit for recombinant GST tagged PFKFB3 is approximately 3ng/ml as a capture antibody.
H00005209-B01P - PFKFB3
Immunofluorescence of purified MaxPab antibody to PFKFB3 on HeLa cell. [antibody concentration 10 ug/ml]
H00005209-B01P - PFKFB3
Western Blot analysis of PFKFB3 expression in transfected 293T cell line (H00005209-T01) by PFKFB3 MaxPab polyclonal antibody.Lane 1: PFKFB3 transfected lysate(57.2 KDa).Lane 2: Non-transfected lysate.
H00005209-D01P - PFKFB3
PFKFB3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PFKFB3 expression in human kidney.
H00005209-D01P - PFKFB3
Western Blot analysis of PFKFB3 expression in transfected 293T cell line (H00005209-T01) by PFKFB3 MaxPab polyclonal antibody.Lane 1: PFKFB3 transfected lysate(59.60 KDa).Lane 2: Non-transfected lysate.
AP15137PU-N - PFKFB3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP15137PU-N - PFKFB3
(LEFT)The anti-PFKFB3 Pab is used in Western blot to detect PFKFB3 in Jurkat cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). (RIGHT)Western blot analysis of anti-PFKFB3 Antibody (C-term) in CEM cell line lysates (35ug/lane). PFKFB3(arrow) was detected using the purified Pab.
TA590185 - PFKFB3
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in Parietal cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590185 - PFKFB3
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PFKFB3 rabbit polyclonal antibody. (TA590185)
TA590185 - PFKFB3
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFKFB3 rabbit polyclonal antibody. (TA590185)
TA590185 - PFKFB3
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKFB3 rabbit polyclonal antibody. (TA590185)
TA590185 - PFKFB3
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & mitochondria.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590185 - PFKFB3
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590185 - PFKFB3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKFB3 (RC206043, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKFB3.
TA590185 - PFKFB3
ELISA: 6 Phosphofructo 2 Kinase Antibody
TP760578 - PFKFB3
TA308467 - PFKFB3
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. PFKFB3 antibody. TA308467 diluted at 1:1000.
TA308467 - PFKFB3
PFKFB3 antibody [C3], C-term detects PFKFB3 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PFKFB3 antibody [C3], C-term (TA308467) dilution: 1:500.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PFKFB3 antibody
  Rabbit polyclonal antibody to PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3)  
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. PFKFB3 antibody. TA308467 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

PFKFB3 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

PFKFB3 - human gene knockout kit via CRISPR
PFKFB3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PFKFB3 - human gene knockout kit via CRISPR
PFKFB3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PFKFB3 - human gene knockout kit via CRISPR
PFKFB3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PFKFB3 - human gene knockout kit via CRISPR
PFKFB3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PFKFB3 (full length, N-term HIS tag, transcript variant 1)
  Purified recombinant protein of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PFKFB3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PFKFB3 overexpression lysate
PFKFB3 overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pfkfb3 (GFP-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pfkfb3 (GFP-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), 200 uL, >10^7 TU/mL  
200 µl / €1,260.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pfkfb3 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pfkfb3 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), 200 uL, >10^7 TU/mL  
200 µl / €1,130.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (4)
  2. Lysate (1)
  3. Primary Antibody (2)
  4. Protein | Growth Factor (1)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (2)
Target
  1. PFKFB3 (3)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (1)
Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. E. coli (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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