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PFKL antibody

Principal name

PFKL antibody

Alternative names for PFKL antibody

Phosphofructokinase 1, 6-phosphofructokinase liver, PFK-B, Phosphohexokinase

SwissProt ID

A1A4J1 (Bovin), P12382 (Mouse), P17858 (Human), P30835 (Rat)

Gene ID

5211 (PFKL), 25741 (Pfkl)

Ncbi ID

9606, NP_002617, NP_002617.3, NM_013190, NM_002626, NM_001002021

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Rb (Rabbit), Eq (Equine), GP (Guinea Pig), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PFKL antibody

Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

PFKL monoclonal antibody (M03), clone 2A9 Western Blot analysis of PFKL expression in HeLa ( Cat # L013V1 ).

H00005211-M03

Immunohistochemistry analyzes of PFK-B Antibody (Cat.-No. AP06677PU-N) in paraffin-embedded human brain tissue.

AP06677PU-N

Western blot analysis of PFK-B Antibody (Cat.-No. AP06677PU-N) in extracts from A549 cells.

AP06677PU-N

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PFKL polyclonal antibody ( Cat # PAB3997 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PAB3997

The PFKL polyclonal antibody ( Cat # PAB3997 ) is used in Western blot to detect PFKL in HepG2 cell lysate.

PAB3997

Western Blot analysis of PFKL expression in transfected 293T cell line (H00005211-T01) by PFKL MaxPab polyclonal antibody.Lane 1: PFKL transfected lysate(90.97 KDa).Lane 2: Non-transfected lysate.

H00005211-B01P

Human HepG2; WB Suggested Anti-PFKL Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. PFKL is supported by BioGPS gene expression data to be expressed in HepG2.; PFKL antibody - middle region (ARP45774_T100) in Human HepG2 cells using Western Blot

ARP45774_T100

Human, Mouse; WB Suggested Anti-PFKL Antibody Titration: 5% Milk. ELISA Titer: dilution: 1:500. Positive Control: human LCL and mouse brains; PFKL antibody - middle region (ARP45774_T100) in Human, Mouse cells using Western Blot

ARP45774_T100

Western blot (WB) analysis of PFK-1 antibody (Cat.-No.: AP20430PU-N) in extracts from A549 and raw264.7 cell.

AP20430PU-N

Western blot analysis of extracts from A549/NIH-3T3 cells, using K6PL Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of PFKL expression in HepG2 (A); A431 (B); Raw264.7 (C); PC12 (D) whole cell lysates.

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308631 diluted at 1:1000

TA308631

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(TA308631) antibody at 1:500 dilution.

TA308631

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(TA308631) antibody at 1:200 dilution.

TA308631

Western blot analysis of extracts from A549 cells and NIH-3T3 cells, using K6PL antibody.The lane on the right is treated with the synthesized peptide.

TA313291

The anti-PFKL Pab (Cat. #TA325027) is used in Western blot to detect PFKL in HepG2 cell lysate.

TA325027

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

TA325027

WB Suggested Anti-PFKL Antibody Titration: 5% Milk; ELISA Titer: dilution: 1: 500; Positive Control: human LCL and mouse brains

TA338906

WB Suggested Anti-PFKL Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate.PFKL is supported by BioGPS gene expression data to be expressed in HepG2

TA338906

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308631	PFKL antibody
	Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
	Rabbit	IgG	Aff - Purified	Hu	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA308669	PFKL antibody
	Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
	Rabbit	IgG	Aff - Purified	Hu	ICC/IF, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
TA313291	PFKL antibody
	Rabbit polyclonal anti-K6PL antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA325027	PFKL (C-term) antibody
	Rabbit polyclonal PFKL Antibody (C-term L684)
	Rabbit	Ig	Purified	Hu, Ms	P, WB	0.4 ml / €415.00
						OriGene Technologies, Inc.
+1 additional image
AP06677PU-N	PFKL antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP20430PU-N	PFKL antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH

Human Lenti ORF Particles

Catalog No.
RC202888L2V	Lenti ORF particles, PFKL (mGFP-tagged) - Human phosphofructokinase, liver (PFKL), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,070.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR210824L2V	Lenti ORF particles, Pfkl (GFP-tagged ORF) - Rat phosphofructokinase, liver (Pfkl), 200 uL, >10^7 TU/mL
		200 µl / €1,580.00
		OriGene Technologies, Inc.

RR210824L1V	Lenti ORF particles, Pfkl (Myc-DDK-tagged ORF) - Rat phosphofructokinase, liver (Pfkl), 200 uL, >10^7 TU/mL
		200 µl / €1,420.00
		OriGene Technologies, Inc.

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