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PFKM antibody

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Principal name

PFKM antibody

Alternative names for PFKM antibody

Phosphofructokinase-M, Phosphohexokinase, Phosphofructokinase 1, PFK1, PFK-1, PFK-A, PFKX, GSD7, 6-phosphofructokinase muscle type, Phosphofructo-1-kinase isozyme A

SwissProt ID

P00511 (Rabit), P08237 (Human), P47858 (Rat), P52034 (Drome), P52784 (Canfa), Q0IIG5 (Bovin), Q2HYU2 (Pig), Q867C9 (Horse)

Gene ID

5213 (PFKM), 65152 (Pfkm)

Ncbi ID

NP_000280.1, 9606, NP_000280, 9986, NM_000289, NM_031715, NM_001166687, NM_001166688

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of PFKM antibody

The PFKM gene encodes the muscle isoform of phosphofructokise (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combitions of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

General readings

Martínez-Costa OH., et al. (2007) FEBS Lett. 581(16):3033-8.
Kim JW., et al. (2004) Mol Cell Biol. 24(13):5923-36.

PAB2141 - PFKM
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PFKM polyclonal antibody ( Cat # PAB2141 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB2141 - PFKM
The PFKM polyclonal antibody ( Cat # PAB2141 ) is used in Western blot to detect PFKM in Ramos cell lysate.
PAB2142 - PFKM
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM polyclonal antibody ( Cat # PAB2142 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB2142 - PFKM
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM polyclonal antibody ( Cat # PAB2142 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB2142 - PFKM
Western blot analysis of PFKM polyclonal antibody ( Cat # PAB2142 ) in HeLa cell line lysate. PFKM ( arrow ) was detected using the purified Polyclonal antibody.
H00005213-M04 - PFKM
PFKM monoclonal antibody (M04), clone 1D1. Western Blot analysis of PFKM expression in K-562 ( Cat # L009V1 ).
H00005213-B01P - PFKM
PFKM MaxPab polyclonal antibody. Western Blot analysis of PFKM expression in rat brain.
H00005213-B01P - PFKM
Western Blot analysis of PFKM expression in transfected 293T cell line (H00005213-T01) by PFKM MaxPab polyclonal antibody.Lane 1: PFKM transfected lysate(85.8 KDa).Lane 2: Non-transfected lysate.
H00005213-D01P - PFKM
PFKM MaxPab rabbit polyclonal antibody. Western Blot analysis of PFKM expression in mouse lung.
H00005213-D01P - PFKM
Western Blot analysis of PFKM expression in transfected 293T cell line (H00005213-T02) by PFKM MaxPab polyclonal antibody.Lane 1: PFKM transfected lysate(85.20 KDa).Lane 2: Non-transfected lysate.
AP15116PU-N - PFKM
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP15116PU-N - PFKM
The PFKM polyclonal antibody is used in Western blot to detect PFKM in Ramos cell lysate.
AP15117PU-N - PFKM
(TOP)Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP15117PU-N - PFKM
Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab
AR09446PU-L - PFKM
AR09446PU-L - PFKM
Recombinant human PFKM, 1-780 aa, His-tagged: 15% SDS-PAGE (3 ug)
ARP61167_P050 - PFKM
Human Fetal heart; WB Suggested Anti-PFKM Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Heart; PFKM antibody - C-terminal region (ARP61167_P050) in Human Fetal heart cells using Western Blot
AM50026PU-N - PFKM
The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human PFKM antibody Cat.-No AM50026PU (1/500~1/2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
TP300702 - PFKM
CPA4490-100ul - PFKM
Western blot analysis of PFKM expression in SHSY5Y (A); A549 (B); Ramos (C) whole cell lysates.
CPA4490-100ul - PFKM
Immunohistochemical analysis of PFKM staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
TA308655 - PFKM
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308655 diluted at 1:2000.
TA308655 - PFKM
Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308655 diluted at 1:5000.
TA332713 - PFKM
Western blot analysis of extracts of various cell lines, using PFKM antibody.
TA343124 - PFKM
WB Suggested Anti-PFKM Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PFKM antibody
  Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308655 diluted at 1:2000. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
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