PGAM2 antibody
Principal name
PGAM2 antibody
Alternative names for PGAM2 antibody
PGAM 2, Phosphoglycerate mutase 2, PGAMM, PGAM-M, BPG-dependent PGAM 2, Phosphoglycerate mutase isozyme M, Muscle-specific phosphoglycerate mutase
Ncbi ID
9606, NP_000281, NP_000281.2, NM_000290, NP_061358.1, NM_018870
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Paraffin Sections (P), Flow Cytometry (F)
Background of PGAM2 antibody
- Kit Component:
- - KN313153G1, Pgam2 gRNA vector 1 in pCas-Guide vector
- KN313153G2, Pgam2 gRNA vector 2 in pCas-Guide vector
- KN313153D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hadjigeorgiou, G.M., et al. Neuromuscul. Disord. 9 (6-7), 399-402 (1999)
Tsujino, S., et al. Am. J. Hum. Genet. 52(3):472-477(1993)
Castella-Escola, J., et al. Gene 91(2):225-232(1990)
Castella-Escola, J., et al. Hum. Genet. 84(2):210-212(1990)
Tsujino, S., et al. J. Biol. Chem. 264(26):15334-15337(1989)
Human Jurkat; WB Suggested Anti-PGAM2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PGAM2 antibody - N-terminal region (ARP48138_P050) in Human Jurkat cells using Western Blot
Human Muscle; WB Suggested Anti-PGAM2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Muscle; PGAM2 antibody - middle region (ARP48139_P050) in Human Muscle cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle reacted with PGAM2 Antibody (N-term) Cat.-No AP53269PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the PGAM2 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PGAM2 Antibody (N-term) Cat.-No AP53269PU-N in mouse testis tissue lysates (35ug/lane). This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).
Western blot analysis of PGAM2 Antibody (N-term) Cat.-No AP53269PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).
Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle stained with PGAM2 Antibody Cat.-No AM50090PU-N (Clone 4E9)
Immunofluorescent staining of HeLa cells using PGAM2 Antibody Cat.-No AM50090PU-N (Clone 4E9)
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2 using PGAM2 Antibody Cat.-No AM50090PU-N (Clone 4E9)
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody, and then analyzed by flow cytometry.
Anti-PGAM2 mouse monoclonal antibody (TA503399) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Anti-PGAM2 mouse monoclonal antibody (TA503400) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Anti-PGAM2 mouse monoclonal antibody (TA503401) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503402)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503402)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503402)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503402)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503402)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503403)
Anti-PGAM2 mouse monoclonal antibody (TA503403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Anti-PGAM2 mouse monoclonal antibody (TA503408) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503408).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503424).
Anti-PGAM2 mouse monoclonal antibody (TA503424) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503425)
Anti-PGAM2 mouse monoclonal antibody (TA503425) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503425).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
HEK293T cells transfected with either RC200701 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PGAM2 antibody(TA503425), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503426).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Anti-PGAM2 mouse monoclonal antibody (TA503440) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PGAM2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Sample (30 ug of whole cell lysate). A: H1299. B: Hela. 12% SDS PAGE. TA308935 diluted at 1:10000.
PGAM2 antibody detects PGAM2 protein by Western blot analysis. A. 50 ug rat muscle lysate/extract. 12 % SDS-PAGE. PGAM2 antibody (TA308935) dilution: 1:10000
Figure from citation: Western Blot of PGAM2 protein level by using anti-PGAM2 antibody in serum samples of DMD patients. View Citation
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PGAM2(TA308935) antibody at 1:200 dilution.
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308935 | PGAM2 antibody |
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| Rabbit polyclonal antibody to PGAM2 (phosphoglycerate mutase 2 (muscle)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Rt | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA503402 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
 |
Mouse | IgG2b | OTI1C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA503424 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) | |||||||
 |
Mouse | IgG2a | OTI2F5 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA503425 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI4E9 (formerly 4E9) | |||||||
 |
Mouse | IgG2a | OTI4E9 | Purified | Hu, Mky, Ms | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA503440 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI4B12 (formerly 4B12) | |||||||
 |
Mouse | IgG2a | OTI4B12 | Purified | Can, Hu, Mky, Ms | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA503399 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI4H1 (formerly 4H1) | |||||||
 |
Mouse | IgG2b | OTI4H1 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503400 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12) | |||||||
 |
Mouse | IgG2b | OTI1E12 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA503401 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11) | |||||||
 |
Mouse | IgG2b | OTI3B11 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503403 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI1C11 (formerly 1C11) | |||||||
 |
Mouse | IgG2b | OTI1C11 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503408 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2) | |||||||
 |
Mouse | IgG2b | OTI1C2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503426 | PGAM2 antibody |
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| PGAM2 mouse monoclonal antibody, clone OTI2H10 (formerly 2H10) | |||||||
 |
Mouse | IgG2a | OTI2H10 | Purified | Can, Hu, Mky, Ms | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| AP53269PU-N | PGAM2 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300701 | PGAM2 |
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| Recombinant protein of human phosphoglycerate mutase 2 (muscle) (PGAM2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424823 | PGAM2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203233L2V | Lenti ORF particles, Pgam2 (GFP-tagged) - Mouse phosphoglycerate mutase 2 (Pgam2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203233L1V | Lenti ORF particles, Pgam2 (Myc-DDK-tagged) - Mouse phosphoglycerate mutase 2 (Pgam2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||