PGD / PGDH antibody
Principal name
PGD / PGDH antibody
Alternative names for PGD / PGDH antibody
6-phosphogluconate dehydrogenase decarboxylating
SwissProt ID
P00349 (Sheep), P00350 (Ecoli), P14332 (Pig), P38720 (Yeast), P41572 (Drome), P52209 (Human), P85968 (Rat), Q17761 (Caeel), Q9DCD0 (Mouse)
Ncbi ID
9606, NP_002622, NP_012053, NP_002622.2, AAH00368, NM_002631, NP_001074743.1, NM_001081274
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Paraffin Sections (P)
Background of PGD / PGDH antibody
- Kit Component:
- - KN313161G1, Pgd gRNA vector 1 in pCas-Guide vector
- KN313161G2, Pgd gRNA vector 2 in pCas-Guide vector
- KN313161D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107(7):3198-203.
Tagen M., et al. (2009) J Immunol. 183(10):6313-9.
Western Blot analysis of PGD expression in transfected 293T cell line (H00005226-T01) by PGD MaxPab polyclonal antibody.Lane 1: PGD transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PGD expression in transfected 293T cell line (H00005226-T01) by PGD MaxPab polyclonal antibody.Lane 1: PGD transfected lysate(53.10 KDa).Lane 2: Non-transfected lysate.
PGD MaxPab rabbit polyclonal antibody. Western Blot analysis of PGD expression in Jurkat.
Recombinant human PGD, 1-483 aa, His-tagged: 15% SDS-PAGE (3 µg)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505578)
Anti-PGD mouse monoclonal antibody (TA505578) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGD(RC201729).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD (RC201729, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGD.
Sample(30 ug whole cell lysate). A:293T. B:MOLT4 . C:Raji . 10% SDS PAGE. TA308104 diluted at 1:1000
PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. PGD antibody [N1N3] (TA308104) dilution: 1:500.
PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGD protein stained by PGD antibody [N1N3] (TA308104) diluted at 1:500. Blue: Hoechst 33342 staining.
Western blot analysis of extracts from A549 cells, using PGD antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PGD Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell PGD is supported by BioGPS gene expression data to be expressed in Jurkat
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505578 | PGD / PGDH antibody |
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| PGD mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
 |
Mouse | IgG1 | OTI2A5 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
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| TA308104 | PGD / PGDH antibody |
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| Rabbit Polyclonal antibody to PGD (phosphogluconate dehydrogenase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA313090 | PGD / PGDH antibody |
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| Rabbit polyclonal anti-PGD antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201729 | PGD - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201729BN | PGD - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201729LP | PGD - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201729RB | PGD - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301729 | PGD / PGDH |
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| Recombinant protein of human phosphogluconate dehydrogenase (PGD) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419202 | PGD overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |