PGM3 antibody
Principal name
PGM3 antibody
Alternative names for PGM3 antibody
PGM 3, Phosphoglucomutase-3, PAGM, AGM1, Acetylglucosamine phosphomutase, Phosphoacetylglucosamine mutase, N-acetylglucosamine-phosphate mutase
SwissProt ID
O95394 (Human), P38628 (Yeast), P57750 (Arath), Q09687 (Schpo), Q9CYR6 (Mouse), Q9UIY2 (Human)
Ncbi ID
9606, NP_056414, NP_001186846.1, AAH01258.1, NM_015599, NP_001157218.1, NM_028352
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PGM3 antibody
- Kit Component:
- - KN313174G1, Pgm3 gRNA vector 1 in pCas-Guide vector
- KN313174G2, Pgm3 gRNA vector 2 in pCas-Guide vector
- KN313174D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml]
Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
PGM3 monoclonal antibody (M01), clone 1E2-1B12 Western Blot analysis of PGM3 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.
PGM3 MaxPab polyclonal antibody. Western Blot analysis of PGM3 expression in human liver.
Western Blot analysis of PGM3 expression in transfected 293T cell line (H00005238-T01) by PGM3 MaxPab polyclonal antibody.Lane 1: PGM3 transfected lysate(59.62 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PGM3 expression in transfected 293T cell line (H00005238-T01) by PGM3 MaxPab polyclonal antibody.Lane 1: PGM3 transfected lysate(59.90 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-PGM3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. PGM3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; PGM3 antibody - N-terminal region (ARP56755_P050) in Human HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-PGM3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate. PGM3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; PGM3 antibody - middle region (ARP56756_P050) in Human HepG2 cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504859)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.
HEK293T cells transfected with either RC201522 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PGM3 antibody(TA504859), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504860)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504860)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504860)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504860)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504860)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.
HEK293T cells transfected with either RC201522 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PGM3 antibody(TA504860), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PGM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504940)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.
HEK293T cells transfected with either RC201522 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PGM3 antibody(TA504940), and then analyzed by flow cytometry.
Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. TA308127 diluted at 1:3000.
Sample (30 ug of whole cell lysate). A: JurKat. 7.5% SDS PAGE. TA308127 diluted at 1:1000.
Immunofluorescence analysis of methanol-fixed HeLa, using PGM3(TA308127) antibody at 1:200 dilution.
WB Suggested Anti-PGM3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysatePGM3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
WB Suggested Anti-PGM3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysatePGM3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504859 | PGM3 antibody |
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| PGM3 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11) | |||||||
 |
Mouse | IgG1 | OTI1A11 | Purified | Hu, Mky, Ms | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
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| TA504860 | PGM3 antibody |
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| PGM3 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) | |||||||
 |
Mouse | IgG1 | OTI1B8 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA308127 | PGM3 antibody |
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| Rabbit Polyclonal antibody to PGM3 (phosphoglucomutase 3) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA504940 | PGM3 antibody |
||||||
| PGM3 mouse monoclonal antibody, clone OTI5C6 (formerly 5C6) | |||||||
 |
Mouse | IgG1 | OTI5C6 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301522 | PGM3 |
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| Recombinant protein of human phosphoglucomutase 3 (PGM3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414446 | PGM3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218001L2V | Lenti ORF particles, Pgm3 (GFP-tagged) - Mouse phosphoglucomutase 3 (Pgm3), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR218001L1V | Lenti ORF particles, Pgm3 (Myc-DDK-tagged) - Mouse phosphoglucomutase 3 (Pgm3), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||