PHKG2 antibody
Principal name
PHKG2 antibody
Alternative names for PHKG2 antibody
PHK-gamma-T, PSK-C3, Phosphorylase b kinase gamma catalytic chain testis/liver isoform, Phosphorylase kinase subunit gamma-2
Ncbi ID
9606, NP_000285, NP_000285.1, NM_000294, NM_001172432, NP_081164.2, NM_026888, NM_080584
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PHKG2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
General readings
Burwinkel,B., et al., (1998) Hum. Mol. Genet. 7 (1), 149-154
Detection limit for recombinant GST tagged PHKG2 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PHKG2 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PHKG2 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PHKG2 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PHKG2 is approximately 10ng/ml as a capture antibody.
Human kidney; PHKG2 antibody - N-terminal region (ARP33072_P050) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-PHKG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. PHKG2 is supported by BioGPS gene expression data to be expressed in HepG2.; PHKG2 antibody - N-terminal region (ARP33072_P050) in Human HepG2 cells using Western Blot
Western Blot analysis of PHKG2 expression in transfected 293T cell line (H00005261-T01) by PHKG2 MaxPab polyclonal antibody.Lane 1: PHKG2 transfected lysate(44.66 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PHKG2 polyclonal antibody ( Cat # PAB2795 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The PHKG2 polyclonal antibody ( Cat # PAB2795 ) is used in Western blot to detect PHKG2 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-PHKG2 Pab (AP13916PU-N) is used in Western blot to detect PHKG2 in mouse kidney tissue lysate.
Western blot analysis of PHKG2 (arrow) using PHKG2 Antibody (N-term) (AP13916PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHKG2 gene (Lane 2) (Origene Technologies).
Western Blot analysis of PHKG2 expression in transfected 293T cell line (H00005261-T01) by PHKG2 MaxPab polyclonal antibody.Lane 1: PHKG2 transfected lysate(46.40 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PHKG2 transfected lysate using anti-PHKG2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PHKG2 MaxPab mouse polyclonal antibody (B01) (H00005261-B01).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PHKG2 mouse monoclonal antibody. (TA800343) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PHKG2 mouse monoclonal antibody. (TA800343) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PHKG2 mouse monoclonal antibody. (TA800343) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHKG2 (RC200597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHKG2.
Anti-PHKG2 mouse monoclonal antibody (TA800354) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PHKG2(RC200597).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHKG2 (RC200597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHKG2.
HEK293T cells transfected with either RC200597 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PHKG2 antibody(TA800354), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PHKG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800355)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PHKG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800355)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PHKG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800355)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PHKG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800355)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PHKG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800355)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHKG2 (RC200597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHKG2.
WB Suggested Anti-PHKG2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysatePHKG2 is supported by BioGPS gene expression data to be expressed in HepG2
Human kidney
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: A549 cells, Hela cells, HepG2 cells, 293T cells, Primary antibody: (PHKG2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (PHKG2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (PHKG2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800343 | PHKG2 antibody |
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| PHKG2 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5) | |||||||
 |
Mouse | IgG2a | OTI2E5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA800355 | PHKG2 antibody |
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| PHKG2 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) | |||||||
 |
Mouse | IgG2b | OTI1F9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA351527 | PHKG2 antibody |
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| Rabbit Polyclonal Anti-PHKG2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA800354 | PHKG2 antibody |
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| PHKG2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) | |||||||
 |
Mouse | IgG2b | OTI2A3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP13916PU-N | PHKG2 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY400111 | PHKG2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY432959 | PHKG2 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215779L2V | Lenti ORF particles, Phkg2 (GFP-tagged) - Mouse phosphorylase kinase, gamma 2 (testis) (Phkg2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR215779L1V | Lenti ORF particles, Phkg2 (Myc-DDK-tagged) - Mouse phosphorylase kinase, gamma 2 (testis) (Phkg2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209049L2V | Lenti ORF particles, Phkg2 (GFP-tagged ORF) - Rat phosphorylase kinase, gamma 2 (testis) (Phkg2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209049L1V | Lenti ORF particles, Phkg2 (Myc-DDK-tagged ORF) - Rat phosphorylase kinase, gamma 2 (testis) (Phkg2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||