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PHLPP2 antibody

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Principal name

PHLPP2 antibody

Alternative names for PHLPP2 antibody

PHLPPL, PHLPP-like, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like

SwissProt ID

Q6ZVD8 (Human), Q8BXA7 (Mouse)

Gene ID

23035 (PHLPP2), 244650 (Phlpp2)

Ncbi ID

9606, NP_055835, NP_055835.2, NM_015020, NP_001116066.2, NM_001122594

Available reactivities

Hu (Human)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PHLPP2 antibody

Kit Component:
- KN313231G1, Phlpp2 gRNA vector 1 in pCas-Guide vector
- KN313231G2, Phlpp2 gRNA vector 2 in pCas-Guide vector
- KN313231D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.
Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.
Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31
Gao MH, Miyanohara A, Feramisco JR, et al. Activation of PH-domain leucine-rich protein phosphatase 2 (PHLPP2) by agonist stimulation in cardiac myocytes expressing adenylyl cyclase type 6. Biochem. Biophys. Res. Commun. 2009; 384:193-8.

PAB7321 - PHLPP2
PHLPPL polyclonal antibody ( Cat # PAB7321 ) ( 0.1 µg/ml ) staining of human brain ( amygdala, hippocampus, substantia nigra ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AP14594PU-N - PHLPP2
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHLPP2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP55482CP-N - PHLPP2

Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody Cat.-No AP55482PU-N at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide Cat.-No. AP55482CP-N.


TA590848 - PHLPP2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHLPP2 (RC212846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLPP2.
TA590848 - PHLPP2
ELISA: PHLPP2 Antibody
TP312846 - PHLPP2
TA303258 - PHLPP2
TA303258 (0.1µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA319840 - PHLPP2
Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
TA319840 - PHLPP2
Immunocytochemistry of PHLPP2 in SW480 cells with PHLPP2 antibody at 2.5 ug/mL.
TA319840 - PHLPP2
Immunofluorescence of PHLPP2 in SW480 cells with PHLPP2 antibody at 5 ug/mL.
TA354024 - PHLPP2
Western Blot analysis of HEK293T cell lysate with anti-PHLPP2 antibody.
TA354024 - PHLPP2
Human prostate adenocarcinoma stained with anti-PHLPP2 antibody.
TA354024 - PHLPP2
Human Normal Prostate stained with anti-PHLLP2 antibody.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PHLPP2 antibody
  Goat Polyclonal Antibody against PHLPPL  
TA303258 (0.1µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

PHLPP2 (C-term) antibody
  Rabbit Polyclonal PHLPP2 Antibody  
Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

PHLPP2 antibody
  Rabbit Polyclonal PHLPP2 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHLPP2 (RC212846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLPP2. Rabbit IgG Purified Hu E, WB
50 µg / €409.00
  OriGene Technologies, Inc.
+1 additional image

PHLPP2 (N-term) antibody
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHLPP2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PHLPP2
  Recombinant protein of human PH domain and leucine rich repeat protein phosphatase-like (PHLPPL)  
PHLPP2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

PHLPP2 overexpression lysate
PHLPP2 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PHLPP2 (mGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), 200 uL, >10^7 TU/mL
Lenti ORF particles, PHLPP2 (mGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), 200 uL, >10^7 TU/mL  
200 µl / €2,680.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, PHLPP2 (Myc-DDK tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2) , 200 uL, >10^7 TU/mL
Lenti ORF particles, PHLPP2 (Myc-DDK tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2) , 200 uL, >10^7 TU/mL  
200 µl / €2,420.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Phlpp2 (GFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Phlpp2 (GFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL  
200 µl / €2,390.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL  
200 µl / €2,150.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Human Lenti ORF Particle (2)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (4)
  5. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (4)
Target
  1. PHLPP2 (5)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (4)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Immunofluorescence (IF) (1)
  4. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • fax: +49-5221-34606-11
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