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Phospholamban antibody

Principal name

Phospholamban antibody

Alternative names for Phospholamban antibody

Cardiac phospholamban, PLB, PLN

SwissProt ID

A4IFH6 (Bovin), P26677 (Chick), P26678 (Human), P61012 (Canfa), P61013 (Pig), P61014 (Mouse), P61015 (Rabit), P61016 (Rat)

Gene ID

5350 (PLN), 18821 (Pln), 64672

Ncbi ID

9606, NP_002658.1, NP_002658, NM_001141927, NM_022707, NM_023129, NM_002667

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of Phospholamban antibody

Phospholamban (PLB/PLN) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains cAMP-dependent protein kinase phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. PLB is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

General readings

1. Møller DV, Pham TT, Gustafsson F, Hedley P, Ersbøll MK, Bundgaard H, Andersen CB, Torp-Pedersen C, Køber L, Christiansen M. The role of Lamin A/C mutations in Danish patients with idiopathic dilated cardiomyopathy.
European journal of heart failure : journal of the Working Group on Heart Failure of the European Society of Cardiology 2009 Nov 11 (11): 1031-5. PMID: 19875404

Western blot of rat heart tissue lysate with PLN polyclonal antibody ( Cat # PAB13686 ).

PAB13686

Immunofluorescence analysis of HuvEc cells, using PLB (Phospho-Ser16+Thr17) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PLB (Ab-16/17) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot with Phospholamban Polyclonal Antibody

AP23398PU-N

Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using PLB-Thr17 Cat.-No AP53332PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

AP53332PU-N

Western blot analysis of PLB-Thr17 Cat.-No AP53332PU-N in Jurkat cell line lysates (35ug/lane). PLB (arrow) was detected using the purified Pab.

AP53332PU-N

Flow cytometric analysis of Jurkat cells using PLB-Thr17 Cat.-No AP53332PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

AP53332PU-N

(0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311243

Western blot analysis of lysate from U266 cell line,using PLN Antibody (N-term)(Cat. #TA328116).TA328116 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

TA328116

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA311243	Phospholamban (N-term) antibody
	Goat Anti-Phospholamban / PLN Antibody
	Goat			Aff - Purified	Bov, Hu, Por, Rt	E, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA328116	Phospholamban antibody
	Rabbit polyclonal anti-PLN antibody (N-term)
	Rabbit		RB44799	Purified	Bov, Hu, Ms, Por, Rb, Rt	WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
AP23398PU-N	Phospholamban (N-term) antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €390.00
							OriGene Technologies GmbH

Mouse Lenti ORF Particles

Catalog No.
MR200019L2V	Lenti ORF particles, Pln (GFP-tagged) - Mouse phospholamban (Pln), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR200019L1V	Lenti ORF particles, Pln (Myc-DDK-tagged) - Mouse phospholamban (Pln), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR200884L2V	Lenti ORF particles, Pln (GFP-tagged ORF) - Rat phospholamban (Pln), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR200884L1V	Lenti ORF particles, Pln (Myc-DDK-tagged ORF) - Rat phospholamban (Pln), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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