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PHOX2A antibody

Principal name

PHOX2A antibody

Alternative names for PHOX2A antibody

ARIX, PMX2A, Paired mesoderm homeobox protein 2A, Paired-like homeobox 2A, ARIX1 homeodomain protein, Aristaless homeobox protein homolog

SwissProt ID

O14813 (Human), Q62066 (Mouse), Q62782 (Rat)

Gene ID

401 (PHOX2A), 11859 (Phox2a), 116648

Ncbi ID

9606, NP_005160, NM_005169, NP_005160.2, NM_008887, NM_053869

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of PHOX2A antibody

PHOX2A contains a paired-like homeodomain most similar to that of the Drosophila aristaless gene product. The encoded protein plays a central role in development of the autonomic nervous system. It regulates the expression of tyrosine hydroxylase and dopamine beta-hydroxylase, two catecholaminergic biosynthetic enzymes essential for the differentiation and maintenance of the noradrenergic neurotransmitter phenotype. PHOX2A has also been shown to regulate transcription of the alpha3 nicotinic acetylcholine receptor gene.The protein encoded by this gene contains a paired-like homeodomain most similar to that of the Drosophila aristaless gene product. The encoded protein plays a central role in development of the autonomic nervous system. It regulates the expression of tyrosine hydroxylase and dopamine beta-hydroxylase, two catecholaminergic biosynthetic enzymes essential for the differentiation and maintenance of the noradrenergic neurotransmitter phenotype. The encoded protein has also been shown to regulate transcription of the alpha3 nicotinic acetylcholine receptor gene. Mutations in this gene have been associated with autosomal recessive congenital fibrosis of the extraocular muscles. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94.

Immunofluorescence of monoclonal antibody to PHOX2A on HeLa cell. [antibody concentration 10 ug/ml]

H00000401-M01

Western blot analysis of PHOX2A over-expressed 293 cell line, cotransfected with PHOX2A Validated Chimera RNAi ( Cat # H00000401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PHOX2A monoclonal antibody (M01), clone 4F6 (Cat # H00000401-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00000401-M01

Western Blot analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.

H00000401-M01

PHOX2A monoclonal antibody (M01), clone 4F6 Western Blot analysis of PHOX2A expression in PC-12 ( Cat # L012V1 ).

H00000401-M01

Immunofluorescence of purified MaxPab antibody to PHOX2A on HeLa cell. [antibody concentration 10 ug/ml]

H00000401-B01

Western Blot analysis of PHOX2A expression in transfected 293T cell line (H00000401-T01) by PHOX2A MaxPab polyclonal antibody.Lane 1: PHOX2A transfected lysate(31.24 KDa).Lane 2: Non-transfected lysate.

H00000401-B01

Human 721_B; WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. PHOX2A is supported by BioGPS gene expression data to be expressed in 721_B.; PHOX2A antibody - N-terminal region (ARP31726_P050) in Human 721_B cells using Western Blot

ARP31726_P050

Immunoprecipitation of PHOX2A transfected lysate using anti-PHOX2A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PHOX2A MaxPab mouse polyclonal antibody (B01) (H00000401-B01).

H00000401-D01

Human Stomach; WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Stomach; PHOX2A antibody - N-terminal region (ARP32081_P050) in Human Stomach cells using Western Blot

ARP32081_P050

Human MCF-7; WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; PHOX2A antibody - C-terminal region (ARP38570_P050) in Human MCF-7 cells using Western Blot

ARP38570_P050

Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody Cat.-No. AP55428CP-N at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

AP55428CP-N

(1ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311322

Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

TA319069

Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

TA319616

Immunohistochemistry of PHOX2A in human brain tissue with PHOX2A antibody at 2.5 ug/mL.

TA319616

Immunofluorescence of PHOX2A in human brain tissue with PHOX2A antibody at 20 ug/mL.

TA319616

WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Stomach

TA329897

WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: MCF7 cell lysate

TA343509

WB Suggested Anti-PHOX2A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysatePHOX2A is supported by BioGPS gene expression data to be expressed in 721_B

TA329397

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA311322	PHOX2A (165-179) antibody
	Goat Anti-PHOX2A (aa165-179) Antibody
	Goat		Aff - Purified	Bov, Can, Hu, Por	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA319616	PHOX2A (C-term) antibody
	Rabbit Polyclonal PHOX2A Antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+2 additional images

Lysates

Catalog No.
LY417472	PHOX2A overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR203444L2V	Lenti ORF particles, Phox2a (GFP-tagged ORF) - Rat paired-like homeobox 2a (Phox2a), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR203444L1V	Lenti ORF particles, Phox2a (Myc-DDK-tagged ORF) - Rat paired-like homeobox 2a (Phox2a), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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