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PIAS4 antibody

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Principal name

PIAS4 antibody

Alternative names for PIAS4 antibody

PIASG, PIAS-gamma, PIASy, E3 SUMO-protein ligase PIAS4, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma

SwissProt ID

Q8N2W9 (Human), Q9JM05 (Mouse)

Gene ID

51588 (PIAS4), 59004 (Pias4)

Ncbi ID

NP_056981.2, 9606, NP_056981, ABM86559.1, NM_021501, NP_067476.2

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PIAS4 antibody

The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

General readings

Zhang,J., et al., (2004) FEBS Lett. 570 (1-3), 97-101

AP06455PU-N - PIAS4
Immunohistochemistry (IHC) analyzes of PIAS4 antibody (Cat.-No.: AP06455PU-N) in paraffin-embedded human lung carcinoma tissue.
AP06455PU-N - PIAS4
Western blot (WB) analysis of PIAS4 antibody (Cat.-No.: AP06455PU-N) in extracts from Jurkat cells.
AP11253PU-N - PIAS4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11253PU-N - PIAS4
Western Blot analysisi using anti-PIASy polyclonal antibody (Cat. #AP11253PU-N) to detect PIASy in HL-60 cell lysate.
AP11254PU-N - PIAS4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11254PU-N - PIAS4
Western blot analysis of PIASy1 polyclonal antibody (Cat. #AP11254PU-N) to detect recombinant GST-PIASy1 in bacterial cell lysate.
ARP33011_P050 - PIAS4
Human Jurkat; WB Suggested Anti-PIAS4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PIAS4 antibody - N-terminal region (ARP33011_P050) in Human Jurkat cells using Western Blot
C0364-1 - PIAS4
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS4 Antibody. The picture on the right is treated with the synthesized peptide.
C0364-1 - PIAS4
Western blot analysis of extracts from Jurkat cells, using PIAS4 Antibody. The lane on the right is treated with the synthesized peptide.
PAB0167 - PIAS4
Western blot analysis of PIAS4 in A ) human liver, B ) mouse liver, C ) rat liver and D ) human Jurkat lysate using PIAS4 polyclonal antibody ( Cat # PAB0167 ) at 5 µg/ml.
PAB2257 - PIAS4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIAS4 polyclonal antibody ( Cat # PAB2257 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB2257 - PIAS4
The PIAS4 polyclonal antibody ( Cat # PAB2257 ) is used in Western blot to detect PIAS4 in HL-60 cell lysate.
PAB2258 - PIAS4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIAS4 polyclonal antibody ( Cat # PAB2258 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB2258 - PIAS4
The PIAS4 polyclonal antibody ( Cat # PAB2258 ) is used in Western blot to detect recombinant GST-PIAS4 in bacterial cell lysate.
TP306748 - PIAS4
TA319932 - PIAS4
Western blot analysis of PIAS4 in rat brain tissue lysate with PIAS4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TA319932 - PIAS4
Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 ug/mL.
TA311668 - PIAS4
Western blot analysis of extracts from Jurkat cells, using PIAS4 antibody.The lane on the right is treated with the synthesized peptide.
TA311668 - PIAS4
Western blot analysis of extracts from JK cells (Lane 2), using PIAS4 Antibody. The lane on the left is treated with synthesized peptide.
TA311668 - PIAS4
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PIAS4 antibody.The picture on the right is treated with the synthesized peptide.
TA331936 - PIAS4
WB Suggested Anti-PIAS4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA347977 - PIAS4
Western blot analysis of PIAS4 expression in Jurkat cell extract

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIAS4 antibody
  Rabbit polyclonal anti-PIAS4 antibody  
Western blot analysis of extracts from Jurkat cells, using PIAS4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

PIAS4 (N-term) antibody
  Rabbit Polyclonal PIAS4 Antibody  
Western blot analysis of PIAS4 in rat brain tissue lysate with PIAS4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

PIAS4 antibody
  Rabbit Polyclonal Anti-PIAS4 Antibody  
Western blot analysis of PIAS4 expression in Jurkat cell extract Rabbit IgG Purified Hu, Ms WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

PIAS4 antibody
Immunohistochemistry (IHC) analyzes of PIAS4 antibody (Cat.-No.: AP06455PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

PIAS4 (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

PIAS4 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIAS4
  Recombinant protein of human protein inhibitor of activated STAT, 4 (PIAS4)  
PIAS4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pias4 (GFP-tagged) - Mouse protein inhibitor of activated STAT 4 (Pias4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pias4 (GFP-tagged) - Mouse protein inhibitor of activated STAT 4 (Pias4), 200 uL, >10^7 TU/mL  
200 µl / €1,200.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pias4 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 4 (Pias4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pias4 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 4 (Pias4), 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (6)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (6)
Target
  1. PIAS4 (7)
Property
  1. C-term (1)
  2. N-term (2)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
Host
  1. Rabbit (6)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Paraffin Sections (P) (3)
  3. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
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