PIBF1 antibody
Principal name
PIBF1 antibody
Alternative names for PIBF1 antibody
PIBF1, C13orf24, PIBF, Progesterone-induced-blocking factor 1
SwissProt ID
Q8WXW3 (Human)
Gene ID
10464 (PIBF1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of PIBF1 antibody
PIBF1 Antibody: PIBF1 is synthesized during pregncy in response to progesterone by T lymphocytes. PIBF1 inhibits arachidonic acid release, controls NK activity, and modifies the cytokine balance exerting an anti-abortive effect. It contains a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization sigl, an ER membrane retention sigl and N-glycosylation and phosphorylation sites. PIBF1 is significantly higher in healthy pregnt women than in women at risk for premature pregncy termition. Full-length PIBF1 is associated with the nucleus and functions as a transcription factor, whereas secretion of shorter forms which may act as cytokines is induced by activation of the cell.
Immunoperoxidase of monoclonal antibody to C13orf24 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]
Detection limit for recombinant GST tagged C13orf24 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of PIBF1 expression in transfected 293T cell line (H00010464-T01) by PIBF1 MaxPab polyclonal antibody.Lane 1: C13orf24 transfected lysate(83.27 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PIBF1 expression in transfected 293T cell line (H00010464-T02) by PIBF1 MaxPab polyclonal antibody.Lane 1: C13orf24 transfected lysate(83.27 KDa).Lane 2: Non-transfected lysate.
Human 293T; WB Suggested Anti-PIBF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. PIBF1 is supported by BioGPS gene expression data to be expressed in HEK293T.; PIBF1 antibody - C-terminal region (ARP52152_P050) in Human 293T cells using Western Blot
Western blot analysis of PIBF1 in human placenta tissue lysate with PIBF1 antibody at (A) 1 and (B) 2 ug/mL
Immunohistochemistry of PBIF in spleen tissue with PBIF antibody at 5 ug/mL.
Immunofluorescence of PBIF in human spleen tissue with PBIF antibody at 20 ug/mL.
Predicted band size: 90 kDa . Positive control: Hela and 293T cell lysate. Recommended dilution: 1/200-1000
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PIBF1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human lung and colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PIBF1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human lung and colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PIBF1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification: x200)
WB Suggested Anti-PIBF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysatePIBF1 is supported by BioGPS gene expression data to be expressed in HEK293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319821 | PIBF1 antibody |
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| Rabbit Polyclonal PIBF1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA321762 | PIBF1 antibody |
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| Anti-PIBF1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA321763 | PIBF1 antibody |
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| Anti-PIBF1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308744 | PIBF1 |
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| Recombinant protein of human progesterone immunomodulatory binding factor 1 (PIBF1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401910 | PIBF1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |