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PICK1 antibody

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Principal name

PICK1 antibody

Alternative names for PICK1 antibody

PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

SwissProt ID

Q2T9M1 (Bovin), Q62083 (Mouse), Q9EP80 (Rat), Q9NRD5 (Human)

Gene ID

9463 (PICK1), 18693 (Pick1), 84591

Ncbi ID

9606, NP_001034672, NP_001034672.1, NP_001034673, NP_036539, NM_053460, NM_012407

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PICK1 antibody

PDZ domain, but not the AH domain, of PICK1 interacts with the C termini of the GTP-bound forms of ADP-ribosylation factor-1 (ARF1) and ARF3. The interactions with ARF5 and ARF6 are weak, suggesting that the PICK1 interaction is specific for class I ARFs and that it may regulate Golgi-to-endoplasmic reticulum vesicle transport. The PDZ domain of rat Pick1 interacts with the last 10 amino acids of the short C-terminal alternative splice variants of AMPA receptor subunits. It has thus been proposed that E-S-V/I-K-I, a sequence found in these 10 amino acids, is a novel PDZ-binding motif. PRKCA phosphorylates Pick1 efficiently but binds Pick1 in both the phosphorylated and unphosphorylated states. Consistent with a neuronal role for PICK1, the mouse homolog interacts with mouse AMPA glutamate receptors and colocalizes at excitatory synapses in the brain. Metabotropic glutamate receptor-7 (mGluR7) localizes specifically to presynaptic active zones. The extreme C-terminal 3 amino acids of mGluR7 have been shown to interact with the PDZ domain of PICK1. Immunofluorescence microscopy demonstrated that both proteins are localized at excitatory synapses in hippocampal neurons, with clustering of mGluR7 at synapses requires PICK1 C-terminal PDZ-binding residues. Mutant mGluR7 lacking the PDZ-binding residues localized diffusely along axons rather than at the synapse, suggesting a role for Pick1 as a scaffolding molecule at presynaptic sites.

H00009463-A01 - PICK1
PRKCABP polyclonal antibody (A01), Lot # 050921JC01 Western Blot analysis of PRKCABP expression in HL-60 ( Cat # L014V1 ).
H00009463-B01 - PICK1
Western Blot analysis of PICK1 expression in transfected 293T cell line (H00009463-T01) by PICK1 MaxPab polyclonal antibody.Lane 1: PRKCABP transfected lysate(45.65 KDa).Lane 2: Non-transfected lysate.
PAB4631 - PICK1
The PICK1 polyclonal antibody ( Cat # PAB4631 ) is used in Western blot to detect PICK1 in Y-79 cell lysate .
PAB4632 - PICK1
The PICK1 polyclonal antibody ( Cat # PAB4632 ) is used in Western blot to detect PICK1 in mouse pancreas tissue lysate .
PAB4632 - PICK1
The PICK1 polyclonal antibody ( Cat # PAB4632 ) is used in Western blot to detect PICK1 in HeLa cell lysate .
PAB7130 - PICK1
PICK1 polyclonal antibody ( Cat # PAB7130 ) ( 1 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AP13655PU-N - PICK1
The anti-PRKCABP Pab is used in Western blot to detect PRKCABP in Y79 cell lysate.
AP13656PU-N - PICK1
Western blot analysis of anti-PRKCABP Antibody (C-term) in K562 cell line lysates (35ug/lane). PRKCABP(arrow) was detected using the purified Pab.
H00009463-D01 - PICK1
Immunoprecipitation of PICK1 transfected lysate using anti-PICK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRKCABP MaxPab mouse polyclonal antibody (B01) (H00009463-B01).
AR51747PU-N - PICK1
TP303455 - PICK1
TP322869 - PICK1
TP322916 - PICK1
75-040 - PICK1
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.
75-040 - PICK1
Adult rat brain membrane immunoblot.
75-040 - PICK1
Adult rat hippocampus immunohistochemistry
75-040 - PICK1
immunoblot against membrane fractions from adult rat brain (RBM) and hippocampi (MHM) from wild-type (WT) and PICK1 knockout (KO) mice probed with L20/8 TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).
73-040 - PICK1
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.
73-040 - PICK1
Adult rat brain membrane immunoblot.
73-040 - PICK1
Adult rat hippocampus immunohistochemistry
73-040 - PICK1
immunoblot against membrane fractions from adult rat brain (RBM) and hippocampi (MHM) from wild-type (WT) and PICK1 knockout (KO) mice probed with L20/8 TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).
TA303155 - PICK1
TA303155 (1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA309463 - PICK1
IHC analysis of Pick1 in frozen sections of mouse brain extract using TA309463.
TA327062 - PICK1
Western blot analysis of extracts of various cell lines, using PICK1 antibody.
TA327062 - PICK1
Immunohistochemistry of paraffin-embedded human rectum using PICK1 antibody at dilution of 1:200 (200x lens).
TA327062 - PICK1
Immunofluorescence analysis of HeLa cell using PICK1 antibody. Blue: DAPI for nuclear staining.
  1. Quick-Filters:
  2. Highly Validated (2)

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PICK1 antibody
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe. Mouse IgG2a L20/8 Supernatant Hu, Ms, Rt C, ICC/IF, IP, WB
5 ml / €490.00
  Antibodies Incorporated
+3 additional images

Highly validated antibody!

NeuroMab

PICK1 antibody
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe. Mouse IgG2a L20/8 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €570.00
  Antibodies Incorporated
+3 additional images

Highly validated antibody!

NeuroMab

PICK1 antibody
  Goat Polyclonal Antibody against PICK1  
TA303155 (1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

PICK1 antibody
  Mouse Monoclonal anti-PICK1 Antibody  
IHC analysis of Pick1 in frozen sections of mouse brain extract using TA309463. Mouse IgG2a S20-8 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €410.00
  OriGene Technologies, Inc.

PICK1 antibody
  PICK1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PICK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

PICK1 (N-term) antibody
The anti-PRKCABP Pab is used in Western blot to detect PRKCABP in Y79 cell lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

PICK1 (C-term) antibody
Western blot analysis of anti-PRKCABP Antibody (C-term) in K562 cell line lysates (35ug/lane). PRKCABP(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PICK1 (transcript variant 3)
  Purified recombinant protein of Homo sapiens protein interacting with PRKCA 1 (PICK1), transcript variant 3  
PICK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

PICK1 (transcript variant 1)
  Recombinant protein of human protein interacting with PRKCA 1 (PICK1), transcript variant 1  
PICK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

PICK1 (transcript variant 2)
  Recombinant protein of human protein interacting with PRKCA 1 (PICK1), transcript variant 2  
PICK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pick1 (GFP-tagged ORF) - Rat protein interacting with PRKCA 1 (Pick1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pick1 (GFP-tagged ORF) - Rat protein interacting with PRKCA 1 (Pick1), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pick1 (Myc-DDK-tagged ORF) - Rat protein interacting with PRKCA 1 (Pick1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pick1 (Myc-DDK-tagged ORF) - Rat protein interacting with PRKCA 1 (Pick1), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

12 Item(s)

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12 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (7)
  2. Protein | Growth Factor (3)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
  2. Monoclonal (3)
Target
  1. PICK1 (10)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (5)
  4. Canine (Can) (1)
  5. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Frozen sections (C) (3)
  3. Immunoprecipitation (IP) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (5)
  2. Aff - Purified (1)
  3. Supernatant (1)
Host
  1. Mouse (3)
  2. Rabbit (3)
  3. Goat (1)
Images
  1. 1 image (10)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Frozen sections (C) (2)
  3. IHC (2)
  4. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (3)
Source
  1. HEK293 cells (3)
Manufacturer
  1. OriGene Technologies (10)
  2. Antibodies Incorporated (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
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