PIGH antibody
Principal name
PIGH antibody
Alternative names for PIGH antibody
PIG-H, Phosphatidylinositol N-acetylglucosaminyltransferase subunit H, Phosphatidylinositol-glycan biosynthesis class H protein
Ncbi ID
9606, NP_004560, NP_004560.1, NM_004569, NP_084264.1, NM_029988
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of PIGH antibody
- Kit Component:
- - KN313269G1, Pigh gRNA vector 1 in pCas-Guide vector
- KN313269G2, Pigh gRNA vector 2 in pCas-Guide vector
- KN313269D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lamesch, P. et al., Genomics 89(3): 307-315 (2007)
Kinoshita, T. et al., Curr Opin Chem Biol 4(6): 632-638 (2000)
Watanabe, R. et al., EMBO J. 17(4): 877-885 (1998)
Watanabe, R., et al., J. Biol. Chem. 271(43): 26868-26875 (1996)
Ware, R.E. e al., Blood 83(12): 3753-3757 (1994)
Western Blot analysis of PIGH expression in transfected 293T cell line (H00005283-T01) by PIGH MaxPab polyclonal antibody.Lane 1: PIGH transfected lysate(20.68 KDa).Lane 2: Non-transfected lysate.
Western blot analysis in HepG2 cell line lysates (35ug/lane) using PIGH antibody Cat.-No. AP53296PU-N (N-term). This demonstrates the PIGH antibody detected the PIGH protein (arrow).
Immunhistochemistry analysis in human kidney tissue (Formalin-fixed, Paraffin-embedded) using PIGH antibody Cat.-No. AP32320PU-N.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PIGH antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314340 | PIGH antibody |
||||||
| Rabbit polyclonal anti-PIGH antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP32320PU-N | PIGH (C-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, P |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP53296PU-N | PIGH (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310028 | PIGH |
||||
| Recombinant protein of human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417898 | PIGH overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218714L2V | Lenti ORF particles, Pigh (GFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (Pigh), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR218714L1V | Lenti ORF particles, Pigh (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (Pigh), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||