PIGL antibody
Principal name
PIGL antibody
Alternative names for PIGL antibody
PIG-L, N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, Phosphatidylinositol-glycan biosynthesis class L protein
Ncbi ID
9606, NM_004278, NP_004269, NP_004269.1, NP_001034625.1, NM_138901
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of PIGL antibody
- Kit Component:
- - KN313271G1, Pigl gRNA vector 1 in pCas-Guide vector
- KN313271G2, Pigl gRNA vector 2 in pCas-Guide vector
- KN313271D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lamesch, P. et al., Genomics 89(3): 307-315 (2007)
Pottekat, A. et al., J. Biol. Chem. 279(16): 15743-15751 (2004)
Kinoshita, T. et al., Curr Opin Chem Biol 4(6): 632-638 (2000)
Watanabe, R. et al., Biochem. J. 339 (PT 1): 185-192 (1999)
Nakamura, N. et al., J. Biol. Chem. 272(25): 15834-15840 (1997)
Detection limit for recombinant GST tagged PIGL is approximately 1ng/ml as a capture antibody.
Human 721_B; WB Suggested Anti-PIGL Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. PIGL is supported by BioGPS gene expression data to be expressed in 721_B.; PIGL antibody - N-terminal region (ARP46427_P050) in Human 721_B cells using Western Blot
Western blot analysis in WiDr cell line lysates (35ug/lane) using PIGL antibody Cat.-No. AP53297PU-N (N-term). This demonstrates the PIGL antibody detected the PIGL protein (arrow).
WB Suggested Anti-PIGL Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate.PIGL is supported by BioGPS gene expression data to be expressed in 721_B
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP53297PU-N | PIGL (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309339 | PIGL |
||||
| Recombinant protein of human phosphatidylinositol glycan anchor biosynthesis, class L (PIGL) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418094 | PIGL overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211185L2V | Lenti ORF particles, Pigl (GFP-tagged ORF) - Rat phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211185L1V | Lenti ORF particles, Pigl (Myc-DDK-tagged ORF) - Rat phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||