PIK3CD antibody
Principal name
PIK3CD antibody
Alternative names for PIK3CD antibody
PI3-kinase subunit delta, PI3K-delta, PtdIns-3-kinase subunit delta, Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform, PtdIns-3-kinase subunit p110-delta
Gene ID
5293 (PIK3CD)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot)
Background of PIK3CD antibody
- Kit Component:
- - KN214047G1, PIK3CD gRNA vector 1 in pCas-Guide vector
- KN214047G2, PIK3CD gRNA vector 2 in pCas-Guide vector
- KN214047D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIK3CD polyclonal antibody ( Cat # PAB3214 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of PIK3CD polyclonal antibody ( Cat # PAB3214 ) in Jurkat cell lysate. PIK3CD ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PIK3CD polyclonal antibody ( Cat # PAB3215 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PIK3CD polyclonal antibody ( Cat # PAB3215 ) in 293 cell line lysates. PIK3CD Antibody ( N-term ) ( arrow ) was detected using the purified polyclonal antibody.
Dot blot analysis of anti-PIK3CD-pTyr524 Phospho-specific Pab (Cat#AP12879PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Dot blot analysis of anti-PIK3CD-pTyr485 Pab (Cat#AP12908PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Dot blot analysis of PIK3CD (phospho Y524) polyclonal antibody ( Cat # PAB8136 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Western blot analysis of PI3K p110 delta expression in K562 (A); Raji (B); Raw264.7 (C); PC12 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801780)
E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 286-610 of human PIK3CD (NP_005017).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801781)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801781)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801781)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801781)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801781)
E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 286-610 of human PIK3CD (NP_005017).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3CD (RC214047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3CD.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801891)
E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 286-610 of human PIK3CD (NP_005017).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801924)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801924)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801924)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801924)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801924)
E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 286-610 of human PIK3CD (NP_005017).
Western blot analysis of anti-PI3KCD Pab (Cat. #TA325014) in Jurkat cell lysate. PI3KCD (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF image of Hela cell stained with PI3KCD Antibody (C-term)(Cat#TA325014).Hela cells were incubated with PI3KCD primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-PI3KCD Antibody (N-term) Pab (TA325015) in 293 cell line lysates. PI3KCD Antibody (N-term)(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCD Antibody (N-term)(Cat.#TA325015), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of Hela cell stained with PI3KCD Antibody (N-term)(Cat#TA325015).Hela cells were incubated with PI3KCD primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (PIK3CD Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN214047 | PIK3CD - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
