PIP5K1C antibody
Principal name
PIP5K1C antibody
Alternative names for PIP5K1C antibody
PIP5K1-gamma, PIP5KIgamma, PtdIns(4)P-5-kinase 1 gamma, KIAA0589, Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
Ncbi ID
9606, NP_001182662.1, NM_012398, NP_036530, NM_001146687, NM_008844, NM_001009967, NM_001033970
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of PIP5K1C antibody
- Kit Component:
- - KN215828G1, PIP5K1C gRNA vector 1 in pCas-Guide vector
- KN215828G2, PIP5K1C gRNA vector 2 in pCas-Guide vector
- KN215828D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged PIP5K1C is approximately 0.03ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1C polyclonal antibody ( Cat # PAB2132 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The PIP5K1C polyclonal antibody ( Cat # PAB2132 ) is used in Western blot to detect PIP5K1C in 293 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1C polyclonal antibody ( Cat # PAB2133 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of PIP5K1C ( arrow ) using PIP5K1C polyclonal antibody ( Cat # PAB2133 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PIP5K1C gene ( Lane 2 ) ( Origene Technologies ) .
The PIP5K1C polyclonal antibody ( Cat # PAB2133 ) is used in Western blot to detect PIP5K1C in HeLa cell lysate.
Western Blot analysis of PIP5K1C expression in transfected 293T cell line (H00023396-T01) by PIP5K1C MaxPab polyclonal antibody.Lane 1: PIP5K1C transfected lysate(22.66 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PIP5K1C expression in transfected 293T cell line (H00023396-T02) by PIP5K1C MaxPab polyclonal antibody.Lane 1: PIP5K1C transfected lysate(21.60 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-PIP5K1G Pab is used in Western blot to detect PIP5K1G in 293 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
(LEFT) The anti-PIP5K1G Pab is used in Western blot to detect PIP5K1G in HeLa cell lysate. (RIGHT) Western blot analysis of PIP5K1C (arrow) using PIP5K1G Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2) (Origene Technologies).
Western blot analysis of PIP5K1C expression in HEK293 (A); K562 (B) whole cell lysates.
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A431 cells, hela cells, Primary antibody: (PIP5K1C Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (PIP5K1C Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (PIP5K1C Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350917 | PIP5K1C antibody |
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| Rabbit Polyclonal Anti-PIP5K1C Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP14964PU-N | PIP5K1C (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP14965PU-N | PIP5K1C (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP315828 | PIP5K1C |
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| Recombinant protein of human phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (PIP5K1C) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402206 | PIP5K1C overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR221039L2V | Lenti ORF particles, Pip5k1c (GFP-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (Pip5k1c), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||
| MR209646L2V | Lenti ORF particles, Pip5k1c (GFP-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (Pip5k1c), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR221039L1V | Lenti ORF particles, Pip5k1c (Myc-DDK-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (Pip5k1c), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
| MR209646L1V | Lenti ORF particles, Pip5k1c (Myc-DDK-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (Pip5k1c), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200082L2V | Lenti ORF particles, Pip5k1c (GFP-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,380.00
|
|
| OriGene Technologies, Inc. | ||
| RR200081L2V | Lenti ORF particles, Pip5k1c (GFP-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,450.00
|
|
| OriGene Technologies, Inc. | ||
| RR200082L1V | Lenti ORF particles, Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,230.00
|
|
| OriGene Technologies, Inc. | ||
| RR200081L1V | Lenti ORF particles, Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,300.00
|
|
| OriGene Technologies, Inc. | ||