PITX1 antibody
Principal name
PITX1 antibody
Alternative names for PITX1 antibody
Pituitary homeobox 1, BFT, PTX1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1
SwissProt ID
O18400 (Drome), P56673 (Chick), P70314 (Mouse), P78337 (Human), Q99NA7 (Rat), Q9W751 (Xenla)
Ncbi ID
NP_002644, NM_002653, NP_002644.4, NM_053624, 9606, NP_035227.1, NM_011097
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of PITX1 antibody
- Kit Component:
- - KN202570G1, PITX1 gRNA vector 1 in pCas-Guide vector
- KN202570G2, PITX1 gRNA vector 2 in pCas-Guide vector
- KN202570D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chen,Y.N., (2008) World J. Gastroenterol. 14 (2), 292-297
Immunohistochemistry (IHC) analyzes of Pitx1 antibody (Cat.-No.: AP20531PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of Pitx1 antibody (Cat.-No.: AP20531PU-N) in extracts from Jurkat cells.
Human 293T; Host:Rabbit. Target Name:PITX1. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml. PITX1 is supported by BioGPS gene expression data to be expressed in HEK293T.; PITX1 antibody - N-terminal region (ARP32339_P050) in Human 293T cells using Western Blot
Human HeLa; Host:Rabbit. Target Name:PITX1. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. PITX1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; PITX1 antibody - N-terminal region (ARP32339_P050) in Human HeLa cells using Western Blot
Human MCF-7; WB Suggested Anti-PITX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; PITX1 antibody - N-terminal region (ARP32339_P050) in Human MCF-7 cells using Western Blot
Human THP-1; WB Suggested Anti-PITX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; PITX1 antibody - middle region (ARP32340_P050) in Human THP-1 cells using Western Blot
Rat Liver; WB Suggested Anti-Pitx1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Liver; Pitx1 antibody - middle region (ARP33001_P050) in Rat Liver cells using Western Blot
Human Jurkat; WB Suggested Anti-PITX1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; PITX1 antibody - C-terminal region (ARP38176_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of PITX1 expression in transfected 293T cell line (H00005307-T01) by PITX1 MaxPab polyclonal antibody.Lane 1: PITX1 transfected lysate(34.54 KDa).Lane 2: Non-transfected lysate.
PITX1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PITX1 expression in mouse brain.
PITX1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PITX1 expression in human placenta.
Western Blot analysis of PITX1 expression in transfected 293T cell line (H00005307-T01) by PITX1 MaxPab polyclonal antibody.Lane 1: PITX1 transfected lysate(34.10 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi ( Cat # H00005307-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 (Cat # H00005307-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
PITX1 monoclonal antibody (M01), clone 5G4 Western Blot analysis of PITX1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.
PITX1 monoclonal antibody (M02), clone 6D4 Western Blot analysis of PITX1 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of extracts from Jurkat cells and COLO cells, using PITX1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PITX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
Host:Rabbit; Target Name:PITX1; Sample Tissue:Human Hela; Antibody Dilution: 1.0ug/mlPITX1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
Host:Rabbit; Target Name:PITX1; Sample Tissue:Human 293T; Antibody Dilution: 1.0ug/mlPITX1 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-PITX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: THP-1 cell lysate
WB Suggested Anti-PITX1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314342 | PITX1 antibody |
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| Rabbit polyclonal anti-PITX1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP20531PU-N | PITX1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204502L2V | Lenti ORF particles, Pitx1 (GFP-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR204502L1V | Lenti ORF particles, Pitx1 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 1 (Pitx1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212470L2V | Lenti ORF particles, Pitx1 (GFP-tagged ORF) - Rat paired-like homeodomain 1 (Pitx1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212470L1V | Lenti ORF particles, Pitx1 (Myc-DDK-tagged ORF) - Rat paired-like homeodomain 1 (Pitx1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
