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PITX2 antibody

Principal name

PITX2 antibody

Alternative names for PITX2 antibody

Pituitary homeobox 2, ARP1, RGS, RIEG, RIEG1, Solurshin, ALL1-responsive protein ARP1, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor

SwissProt ID

O93385 (Chick), P97474 (Mouse), Q99697 (Human), Q9PWR3 (Xenla), Q9R0W1 (Rat), Q9W5Z2 (Danre)

Gene ID

5308 (PITX2), 18741 (Pitx2), 54284

Ncbi ID

9606, NP_000316, NP_700475, AAH13998, NM_000325, NM_153426, NM_153427, NP_700476, NP_000316.2, NP_001035967.1, NM_011098, NM_001042502, NM_019334, NM_001042505

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of PITX2 antibody

The PITX2 gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in PITX2 are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein is involved in the development of the eye, tooth and abdomil organs. It also acts as a transcriptiol regulator involved in basal and hormone-regulated activity of prolactin. This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the termil differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdomil organs, and acts as a transcriptiol regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determition of left-right asymmetry during development. Altertively spliced transcript variants encoding distinct isoforms have been described.

General readings

Martin,D.M., et al., (2004) Dev. Biol. 267 (1), 93-108

Detection limit for recombinant GST tagged PITX2 is approximately 0.03ng/ml as a capture antibody.

H00005308-M01

PITX2 monoclonal antibody (M02), clone 3D2 Western Blot analysis of PITX2 expression in K-562 ( Cat # L009V1 ).

H00005308-M02

PITX2 monoclonal antibody (M02), clone 3D2 Western Blot analysis of PITX2 expression in Hela NE ( Cat # L013V3 ).

H00005308-M02

Human Intestine; PITX2 antibody - N-terminal region (ARP35634_T100) in Human Intestine cells using Immunohistochemistry

ARP35634_T100

Human HepG2; WB Suggested Anti-PITX2 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; PITX2 antibody - N-terminal region (ARP35634_T100) in Human HepG2 cells using Western Blot

ARP35634_T100

Western blot analysis of PITX2 polyclonal antibody ( Cat # PAB3073 ) in CEM cell line lysates ( 35 µg/lane ) . PITX2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .

PAB3073

Proximity Ligation Analysis (PLA) of protein-protein interactions between PITX2 and CTNNB1. HeLa cells were stained with anti-PITX2 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00005308-D01P

PITX2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PITX2 expression in human liver.

H00005308-D01P

Western Blot analysis of PITX2 expression in transfected 293T cell line (H00005308-T01) by PITX2 MaxPab polyclonal antibody.Lane 1: PITX2 transfected lysate(30.30 KDa).Lane 2: Non-transfected lysate.

H00005308-D01P

Human COLO205; WB Suggested Anti-PITX2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: COLO205 cell lysate. PITX2 is supported by BioGPS gene expression data to be expressed in COLO205.; PITX2 antibody - N-terminal region (ARP32431_P050) in Human COLO205 cells using Western Blot

ARP32431_P050

Human Heart; WB Suggested Anti-PITX2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:31250. Positive Control: Human heart; PITX2 antibody - N-terminal region (ARP32432_P050) in Human Heart cells using Western Blot

ARP32432_P050

TP760239

TP760287

Western blot analysis of anti-Pilx2 Pab (Cat.#TA324544) in CEM cell line lysates (35ug/lane). Pilx2(arrow) was detected using the purified Pab.

TA324544

IF image of Hela cell stained with PITX2 Antibody (C-term)(Cat#TA324544/SA070209B).Hela cells were incubated with PITX2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PITX2 immunoreactivity is localized to Nucleus significantly.

TA324544

WB Suggested Anti-PITX2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: COLO205 cell lysatePITX2 is supported by BioGPS gene expression data to be expressed in COLO205

TA330536

WB Suggested Anti-PITX2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:31250; Positive Control: Human heart

TA330537

WB Suggested Anti-PITX2 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate

TA339054

Human Intestine

TA339054

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A375 cells, 293T cells, SKOV3 cells, Primary antibody: (PITX2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

TA349578

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA324544	PITX2 (C-term) antibody
	Rabbit polyclonal PITX2 Antibody (C-term)
	Rabbit	Ig	Aff - Purified	Hu	WB	0.4 ml / €415.00
						OriGene Technologies, Inc.
+1 additional image
TA349578	PITX2 antibody
	Rabbit Polyclonal Anti-PITX2 Antibody
	Rabbit	IgG	Purified	Hu, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP760239	PITX2 (full length, N-term HIS tag, transcript variant 2)
	Recombinant protein of human paired-like homeodomain 2 (PITX2), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.
TP760287	PITX2 (full length, N-term HIS tag, transcript variant 1)
	Recombinant protein of human paired-like homeodomain 2 (PITX2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY400123	PITX2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY407091	PITX2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY407092	PITX2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR200358L2V	Lenti ORF particles, Pitx2 (GFP-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR200357L2V	Lenti ORF particles, Pitx2 (GFP-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR200358L1V	Lenti ORF particles, Pitx2 (Myc-DDK-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

RR200357L1V	Lenti ORF particles, Pitx2 (Myc-DDK-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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PITX2 antibody

Principal name

Alternative names for PITX2 antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of PITX2 antibody

General readings

Primary Antibodies

PITX2 (C-term) antibody

PITX2 antibody

Proteins & Growth Factors

PITX2 (full length, N-term HIS tag, transcript variant 2)

PITX2 (full length, N-term HIS tag, transcript variant 1)

Lysates

PITX2 overexpression lysate

PITX2 overexpression lysate

PITX2 overexpression lysate

Rat Lenti ORF Particles

Lenti ORF particles, Pitx2 (GFP-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Pitx2 (GFP-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Pitx2 (Myc-DDK-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Pitx2 (Myc-DDK-tagged ORF) - Rat paired-like homeodomain 2 (Pitx2), transcript variant 2, 200 uL, >10^7 TU/mL

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