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PLA2G4A antibody

Principal name

PLA2G4A antibody

Alternative names for PLA2G4A antibody

cPLA2, phospholipase A2, Cytosolic phospholipase A2, Phospholipase A2 group IVA

SwissProt ID

A4IFJ5 (Bovin), P47712 (Human), P47713 (Mouse), P49147 (Chick), P50393 (Rat), Q7T0T9 (Xenla), Q9TT38 (Rabit)

Gene ID

5321 (PLA2G4A), 18783 (Pla2g4a)

Ncbi ID

9606, 10090, NP_077734, NP_077734.1, NM_024420, NM_008869

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of PLA2G4A antibody

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995).

General readings

1. Singh NK et al. Both Kdr and Flt1 play a vital role in hypoxia-induced Src-PLD1-PKC?-cPLA(2) activation and retinal neovascularization. Blood 121:1911-23 (2013). Human .PubMed: 23319572
2. Estevez B et al. LIM kinase-1 selectively promotes glycoprotein Ib-IX-mediated TXA2 synthesis, platelet activation, and thrombosis. Blood 121:4586-94 (2013). Mouse .PubMed: 23620575
3. Ma L et al. An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model. Mol Pain 9:29 (2013). IHC-Fr ; Mouse .PubMed: 23773289
4. Zhang Q et al. Activation of Cytosolic Phospholipase A2 Downstream of the Src-Phospholipase D1 (PLD1)-Protein Kinase C {gamma} (PKC{gamma}) Signaling Axis Is Required for Hypoxia-induced Pathological Retinal Angiogenesis. J Biol Chem 286:22489-98 (2011). WB ; Human .PubMed: 21536681
5. Kishimoto K et al. Cytosolic phospholipase A2 alpha amplifies early cyclooxygenase-2 expression, oxidative stress and MAP kinase phosphorylation after cerebral ischemia in mice. J Neuroinflammation 7:42 (2010). WB ; Mouse .PubMed: 20673332
6. Manosalva I & González A Aging alters histone H4 acetylation and CDC2A in mouse germinal vesicle stage oocytes. Biol Reprod 81:1164-71 (2009).PubMed: 19641176

Detection limit for recombinant GST tagged PLA2G4A is approximately 0.3ng/ml as a capture antibody.

H00005321-M01

Western blot analysis of PLA2G4A Antibody (Center) (AP17650PU-N) in NCI-H460 cell line lysates (35ug/lane). PLA2G4A (arrow) was detected using the purified Pab.

AP17650PU-N

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml 30', using c-PLA2 (Phospho-Ser505) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using c-PLA2 (Ab-505) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using c-PLA2 (Ab-505) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 (Ab-505) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of PLA2G4A (pS505) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PLA2G4A (pS505) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of PLA2G4A (pS505) expression in HeLa lambda protein phosphatase-treated (A); NIH3T3 lambda protein phosphatase-treated (B) whole cell lysates.

Immunohistochemical analysis of PLA2G4A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PLA2G4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of PLA2G4A expression in HeLa (A); THP1 (B); NIH3T3 (C) whole cell lysates.

Western blot analysis of PLA2G4A expression in A549 (A); HeLa (B); mouse kidney (C) whole cell lysates.

TP320972

(1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311521

Western blot analysis of c-PLA2 expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA326028

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (PLA2G4A Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

TA349824

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (PLA2G4A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

TA349824

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (PLA2G4A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

TA349824

Western blot analysis of c-PLA2 phosphorylation expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA326029

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA311521	PLA2G4A antibody
	Goat Anti-PLA2G4A Antibody
	Goat		Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA326028	PLA2G4A antibody
	Rabbit Polyclonal c-PLA2 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA326029	PLA2G4A antibody
	Rabbit Polyclonal c-PLA2 (Ser505) Antibody (Phospho-specific)
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA349824	PLA2G4A antibody
	Rabbit Polyclonal Anti-PLA2G4A Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
AP17650PU-N	PLA2G4A antibody
	Rabbit			Hu	WB	0.4 ml / €415.00
						OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP320972	PLA2G4A
	Recombinant protein of human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY402988	PLA2G4A overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210449L2V	Lenti ORF particles, Pla2g4a (GFP-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), 200 uL, >10^7 TU/mL
		200 µl / €1,540.00
		OriGene Technologies, Inc.

MR210449L1V	Lenti ORF particles, Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), 200 uL, >10^7 TU/mL
		200 µl / €1,380.00
		OriGene Technologies, Inc.

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