PLCG1 antibody
Principal name
PLCG1 antibody
Alternative names for PLCG1 antibody
PLC1, Phospholipase C-gamma-1, PLC-gamma-1, PLC gamma1 Phospholipase C-II, PLC-II, PLC-148, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, Phosphoinositide phospholipase C-gamma-1
Ncbi ID
NP_877963, 9606, NP_002651.2, NP_002651, NP_067255.2, NM_021280, NM_182811, NM_002660
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of PLCG1 antibody
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC γ1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation, and may have a protective function during cellular response to oxidative stress.
Western blot analysis of PLCγ 1&2 expression in Jurkat cell lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and PLCG1 HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to PLCG1 on HeLa cell. [antibody concentration 40 ug/ml]
PLCG1 monoclonal antibody (M01), clone 2A2 Western Blot analysis of PLCG1 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PLCG1 is approximately 0.1ng/ml as a capture antibody.
Figure 1. Western blot analysis of extract from HL60 cells, untreated or treated with EGF using PLCG1 antibody (Phospho-Tyr783) Cat.-No AP02395PU-N/PU-S.
Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200 ng/ml, 10 min), using PLCγ1 antibody AP02658PU (Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody AP02395PU (Lane 3 and 4).
Immunohistochemistry (IHC) analyzes of PLCG1 antibody (Cat.-No AP06287PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of PLC γ1 antibody (Cat.-No.: AP06287PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:HEK293T whole cell lysate
Lane1:Hela whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:HEK293T whole cell lysate
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-gamma 1 Antibody AP08095PU.
Figure 2. Western blot analysis of extracts from 293 cell using PLC-gamma 1 Antibody AP08095PU (Lane 1 and 2).
Immunoblot analysis of cell lysates. Lane 1 : HepG2 cell lysate, lane 2 : 293T cell lysate and lane 3 : mouse brain.
Immunoblot analysis of cell lysates. Lane 1 : HepG2 cell lysate, lane 2 : 293T cell lysate and lane 3 : mouse brain.
Western blot analysis of PLCG1 immunoprecipitates from human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). Immunoprecipitation was performed with monoclonal anti-PLCG1. The blots were probed with PLCG1 monoclonal antibody, clone M156 ( Cat # MAB1377 ).
Western blot analysis of PLCG1 immunoprecipitates from human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 min (lane 2). The blots were probed with PLCG1 (phospho Y775) polyclonal antibody ( Cat # PAB7929 ).
Formalin-Fixed, Paraffin-Embedded Human Colon, lymphocytes PLCG1 antibody Cat.-No AP08580PU-N at 1/25-1/100 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using PLCG1 pTyr771 Antibody (#AP09485PU, Red).
Western Blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-gamma1 (pTyr771) antibody
Detection of endogenous Phospholipase C gamma: Whole cell lysates of EGF stimulated serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM20220PU-N PLCG antibody (Clone 2D5) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5Lane 5: HaCaTLane 6: PC3Lane 7: HeLaLane 8: HepG2
Immunohistochemistry analyzes of PLC γ1 antibody (AP20282PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot analysis of PLC γ1 antibody (AP20282PU-N) in extracts from COS7 cells.
Immunofluorescent analysis of PLC gamma 1 (pY1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PLC gamma 1 (pY1253) expression in MCF7 EGF-treated (A); A431 (B); mouse brain (C); mouse liver (D); H9C2 EGF-treated (E) whole cell lysates.
Immunohistochemical analysis of PLC gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PLC gamma 1 expression in HEK293T (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.
Immunofluorescent analysis of PLC gamma 1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PLC gamma 1 expression in HeLa (A); Raw264.7 (B); mouse colon (C); rat colon (D) whole cell lysates.
Immunohistochemical analysis of PLC gamma 1 (pY783) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PLC gamma 1 (pY783) expression in Jurkat pervanadate-treated (A); NIH3T3 LPS-treated (B); PC12 H2O2-treated (C) whole cell lysates.
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using PLCG1 (Ab-771) antibody (#TA313086, Line 1 and 2).The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells (Lane 2), and JK cells (Lane 3), using PLCG1 (Ab-771) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLCG1 (Ab-771) antibody (#TA313086).The picture on the right is treated with the synthesized peptide.
Predicted band size: 149 kDa . Positive control: Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Hela and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of PLCG1 expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of PLCG1 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of PLCG1 phosphorylation expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of PLCG1 phosphorylation expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP09485PU-N | PLCG1 pTyr771 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AP09485PU-S | PLCG1 pTyr771 antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
