PLCL2 antibody
Principal name
PLCL2 antibody
Alternative names for PLCL2 antibody
PLC-L2, PLCE2, Phospholipase C-epsilon-2, Phospholipase C epsilon 2, PLC-epsilon-2, Phospholipase C-L2, Inactive phospholipase C-like protein 2, KIAA1092
Ncbi ID
9606, NP_001137854, NP_001137854.1, NM_015184, NP_055999, NP_038908.2, NM_013880, NM_001144382
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of PLCL2 antibody
- Kit Component:
- - KN313425G1, Plcl2 gRNA vector 1 in pCas-Guide vector
- KN313425G2, Plcl2 gRNA vector 2 in pCas-Guide vector
- KN313425D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002)
Otsuki, M., et al. Biochem. Biophys. Res. Commun. 266(1):97-103(1999)
Detection limit for recombinant GST tagged PLCL2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of PLCL2 expression in transfected 293T cell line (H00023228-T01) by PLCL2 MaxPab polyclonal antibody.Lane 1: PLCL2 transfected lysate(110.11 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PLCL2 Antibody (C-term) Cat.-No AP53339PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) Cat.-No AP53339PU-N with NCI-H460 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of PLCL2 Antibody (C-term) Cat.-No AP53339PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).
Flow cytometric analysis of NCI-H460 cells using PLCL2 Antibody (C-term) Cat.-No AP53339PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP53339PU-N | PLCL2 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307303 | PLCL2 (transcript variant 2) |
||||
| Recombinant protein of human phospholipase C-like 2 (PLCL2), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414738 | PLCL2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC227478L2V | Lenti ORF particles, PLCL2 (mGFP-tagged)-Human phospholipase C-like 2 (PLCL2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,380.00
|
|
| OriGene Technologies, Inc. | ||
| RC227478L1V | Lenti ORF particles, PLCL2 (Myc-DDK-tagged)-Human phospholipase C-like 2 (PLCL2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,140.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211673L2V | Lenti ORF particles, Plcl2 (GFP-tagged) - Mouse phospholipase C-like 2 (Plcl2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,060.00
|
|
| OriGene Technologies, Inc. | ||
| MR211673L1V | Lenti ORF particles, Plcl2 (Myc-DDK-tagged) - Mouse phospholipase C-like 2 (Plcl2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,860.00
|
|
| OriGene Technologies, Inc. | ||