PLEKHM1 antibody
Principal name
PLEKHM1 antibody
Alternative names for PLEKHM1 antibody
KIAA0356, Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1
Ncbi ID
9606, NP_055613.1, NP_055613, NM_014798, Q9Y4G2, NP_898855.1, NM_183034
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of PLEKHM1 antibody
PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.
General readings
Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010)
Del Fattore, A., et al. J. Bone Miner. Res. 23(3):380-391(2008)
Van Wesenbeeck, L., et al. J. Clin. Invest. 117(4):919-930(2007)
Hartel-Schenk, S., et al. Glycoconj. J. 18 (11-12), 915-923 (2001)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306873 | PLEKHM1 antibody |
||||||
| Rabbit Polyclonal PLEKHM1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AP53352PU-N | PLEKHM1 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP315546 | PLEKHM1 |
||||
| Recombinant protein of human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415007 | PLEKHM1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211592L2V | Lenti ORF particles, Plekhm1 (GFP-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,000.00
|
|
| OriGene Technologies, Inc. | ||
| MR211592L1V | Lenti ORF particles, Plekhm1 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,790.00
|
|
| OriGene Technologies, Inc. | ||
