PMVK antibody
Principal name
PMVK antibody
Alternative names for PMVK antibody
Phosphomevalonate kinase, PMKase, PMKI, HUMPMKI
Ncbi ID
NP_006547, 9606, NP_006547.1, NP_081060.2, NM_026784, NM_027348, NM_006556
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)
Background of PMVK antibody
- Kit Component:
- - KN313526G1, Pmvk gRNA vector 1 in pCas-Guide vector
- KN313526G2, Pmvk gRNA vector 2 in pCas-Guide vector
- KN313526D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Cho Y K., et al. (2001) J Biol chem. 278:4510-4515.
Hogenboom S., et al. (2004) J Lipid Res. 45:697-705.
PMVK MaxPab polyclonal antibody. Western Blot analysis of PMVK expression in human kidney.
Western Blot analysis of PMVK expression in transfected 293T cell line (H00010654-T01) by PMVK MaxPab polyclonal antibody.Lane 1: PMVK transfected lysate(21.12 KDa).Lane 2: Non-transfected lysate.
PMVK MaxPab polyclonal antibody. Western Blot analysis of PMVK expression in human kidney.
Western Blot analysis of PMVK expression in transfected 293T cell line (H00010654-T01) by PMVK MaxPab polyclonal antibody.Lane 1: PMVK transfected lysate(21.12 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle sections, stain PMVK in cytoplasm DAB chromogenic reaction.
Recombinant human PMVK, 1- 192 aa, His-tagged
Immunohistochemical analysis of paraffin embedded rat skeletal muscle sections, staining PMVK in cytoplasm, DAB chromeric reaction
Western blot analysis of cardiac muscle tissue lysis using PMKV antibody
Anti-PMVK mouse monoclonal antibody (TA503447) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PMVK(RC202867).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMVK (RC202867, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMVK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMVK (RC202867, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMVK.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PMVK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-PMVK mouse monoclonal antibody (TA503449) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PMVK(RC202867).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMVK (RC202867, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMVK.
HEK293T cells transfected with either RC202867 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PMVK antibody(TA503449), and then analyzed by flow cytometry.
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Jukat cell lysate Lane 4. : MCF7 cell lysate Lane 5. : A549 cell lysate
ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503447 | PMVK antibody |
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| PMVK mouse monoclonal antibody, clone OTI1C12 (formerly 1C12) | |||||||
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Mouse | IgG2a | OTI1C12 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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| TA503448 | PMVK antibody |
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| PMVK mouse monoclonal antibody, clone OTI2D1 (formerly 2D1) | |||||||
 |
Mouse | IgG2b | OTI2D1 | Purified | Hu, Ms | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503449 | PMVK antibody |
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| PMVK mouse monoclonal antibody, clone OTI2F9 (formerly 2F9) | |||||||
 |
Mouse | IgG2a | OTI2F9 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM50615PU-N | PMVK antibody |
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 |
Mouse | AT21C10 | Purified | Hu | E, F, ICC/IF, WB |
0.1 ml /
€330.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM50615PU-S | PMVK antibody |
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|
Mouse | AT21C10 | Purified | Hu | E, F, ICC/IF, WB |
50 µl /
€220.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP23367PU-N | PMVK antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€390.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302867 | PMVK |
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| Recombinant protein of human phosphomevalonate kinase (PMVK) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720221 | PMVK |
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| Recombinant protein of human phosphomevalonate kinase (PMVK) | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR212704L2V | Lenti ORF particles, Pmvk (GFP-tagged) - Mouse phosphomevalonate kinase (Pmvk), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR212706L2V | Lenti ORF particles, Pmvk (GFP-tagged) - Mouse phosphomevalonate kinase (Pmvk), transcript variant 2, 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR212704L1V | Lenti ORF particles, Pmvk (Myc-DDK-tagged) - Mouse phosphomevalonate kinase (Pmvk), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR212706L1V | Lenti ORF particles, Pmvk (Myc-DDK-tagged) - Mouse phosphomevalonate kinase (Pmvk), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||