PNKP antibody
Principal name
PNKP antibody
Alternative names for PNKP antibody
Bifunctional polynucleotide phosphatase/kinase, Polynucleotide kinase-3'-phosphatase, DNA 5'-kinase/3'-phosphatase
Gene ID
11284 (PNKP)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PNKP antibody
- Kit Component:
- - KN207551G1, PNKP gRNA vector 1 in pCas-Guide vector
- KN207551G2, PNKP gRNA vector 2 in pCas-Guide vector
- KN207551D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Jilani A, Ramotar D, Slack C, Ong C, Yang XM, Scherer SW, Lasko DD.
Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
J Biol Chem. 1999 Aug 20;274(34):24176-86.
PMID: 10446192
PNKP polyclonal antibody (A01), Lot # 051017JC01. Western Blot analysis of PNKP expression in Raw 264.7.
PNKP polyclonal antibody (A01), Lot # 051017JC01. Western Blot analysis of PNKP expression in PC-12.
PNKP MaxPab polyclonal antibody. Western Blot analysis of PNKP expression in human spleen.
Western Blot analysis of PNKP expression in transfected 293T cell line (H00011284-T01) by PNKP MaxPab polyclonal antibody.Lane 1: PNKP transfected lysate(57.31 KDa).Lane 2: Non-transfected lysate.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney.
Western blot using PNKP polyclonal antibody ( Cat # PAB11245 ) shows detection of a 57 kDa band corresponding to :Lane 1, human PNKP in a Y190 yeast cell lysate.Lane 2, Y190 yeast cell lysate + human PNKP ( Gal DNA BP ).Lane 3, EM9 XH Chinese hamster ovary cell lysate.Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.Lane 5, a HeLa cell lysate.Approximately 10 µg of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.
Transfected 293T; WB Suggested Anti-PNKP Antibody. . Titration: 1.0 ug/ml. . Positive Control: Transfected 293T; PNKP antibody - N-terminal region (ARP52311_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-PNKP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; PNKP antibody - middle region (ARP52312_P050) in Transfected 293T cells using Western Blot
WB using Anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody.
WB Suggested Anti-PNKP Antibody; Titration: 1.0 ug/ml; Positive Control: Transfected 293T
WB Suggested Anti-PNKP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319448 | PNKP antibody |
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| Rabbit polyclonal anti-PNK antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Chimp, Hu | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP07618PU-N | PNKP (Internal) antibody |
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 |
Rabbit | Aff - Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| AP16044PU-N | PNKP (C-term) antibody |
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 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307551 | PNKP |
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| Recombinant protein of human polynucleotide kinase 3'-phosphatase (PNKP) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416093 | PNKP overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |