PNPO antibody
Principal name
PNPO antibody
Alternative names for PNPO antibody
Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PNPO antibody
- Kit Component:
- - KN200133G1, PNPO gRNA vector 1 in pCas-Guide vector
- KN200133G2, PNPO gRNA vector 2 in pCas-Guide vector
- KN200133D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Musayev, F.N., et al. J. Biol. Chem. 284(45):30949-30956(2009)
Khayat, M., et al. Mol. Genet. Metab. 94(4):431-434(2008)
Song, H., et al. Schizophr. Res. 97 (1-3), 264-270 (2007)
Mills, P.B., et al. Hum. Mol. Genet. 14(8):1077-1086(2005)
Kang, J.H., et al. Eur. J. Biochem. 271(12):2452-2461(2004)
Musayev, F.N., et al. Protein Sci. 12(7):1455-1463(2003)
PNPO polyclonal antibody (A01), Lot # 060529JCS1 Western Blot analysis of PNPO expression in MES-SA/Dx5 ( Cat # L021V1 ).
Western Blot analysis of PNPO expression in transfected 293T cell line (H00055163-T01) by PNPO MaxPab polyclonal antibody.Lane 1: PNPO transfected lysate(28.71 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-PNPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; PNPO antibody - N-terminal region (ARP58515_P050) in Human Muscle cells using Western Blot
Recombinant human PNPO, 57-261aa, His-tagged
The extracts of HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis:The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis of PNPO Antibody (C-term) Cat.-No AP53372PU-N in 293 cell line lysates (35ug/lane). PNPO (arrow) was detected using the purified Pab.
Anti-PNPO mouse monoclonal antibody (TA503178) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO(RC200133).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
HEK293T cells transfected with either RC200133 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNPO antibody(TA503178), and then analyzed by flow cytometry.
Anti-PNPO mouse monoclonal antibody (TA503249) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO(RC200133).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PNPO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Flow cytometric Analysis of Jurkat cells, using anti-PNPO antibody(TA503506),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PNPO antibody(TA503506),(Red), compared to a nonspecific negative control antibody,(Blue).
WB Suggested Anti-PNPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503356 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) | |||||||
 |
Mouse | IgG2b | OTI1H9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA503506 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI1G9 (formerly 1G9) | |||||||
 |
Mouse | IgG2a | OTI1G9 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA503178 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | |||||||
 |
Mouse | IgG1 | OTI2F8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503249 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI3B3 (formerly 3B3) | |||||||
 |
Mouse | IgG2a | OTI3B3 | Purified | Can, Hu, Mky, Rt | ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM39001PU-N | PNPO antibody |
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 |
Mouse | IgG1 | AT2C7 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM39001PU-S | PNPO antibody |
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|
Mouse | IgG1 | AT2C7 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP53372PU-N | PNPO (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300133 | PNPO |
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| Recombinant protein of human pyridoxamine 5'-phosphate oxidase (PNPO) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203425L2V | Lenti ORF particles, Pnpo (GFP-tagged) - Mouse pyridoxine 5'-phosphate oxidase (Pnpo), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203425L1V | Lenti ORF particles, Pnpo (Myc-DDK-tagged) - Mouse pyridoxine 5'-phosphate oxidase (Pnpo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211848L2V | Lenti ORF particles, Pnpo (GFP-tagged ORF) - Rat pyridoxine 5'-phosphate oxidase (Pnpo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211848L1V | Lenti ORF particles, Pnpo (Myc-DDK-tagged ORF) - Rat pyridoxine 5'-phosphate oxidase (Pnpo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||