Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » POFUT2 antibody

POFUT2 antibody

(reset)

Principal name

POFUT2 antibody

Alternative names for POFUT2 antibody

C21orf80, FUT13, KIAA0958, O-FucT-2, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2

SwissProt ID

Q8VHI3 (Mouse), Q8WR51 (Caeel), Q9W589 (Drome), Q9Y2G5 (Human), Q9Y2G5-1

Gene ID

23275 (POFUT2), 80294 (Pofut2)

Ncbi ID

NP_056042, NP_598368, NP_056042.1, 9606, NM_133635, NM_015227, NM_030262, NP_084538.2

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of POFUT2 antibody

Fucose is typically found as a termil modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]. Transcript Variant: This variant (3) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (C) is longer and differs in the C-terminus compared to isoform A. ##Evidence-Data-START## Transcript exon combition :: AY066015.2, BC064623.1 [ECO:0000332] Rseq introns :: single sample supports all introns ERS025095 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Menzel, O., et al. Genomics 84(2):320-330(2004)
Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003)

AP53376PU-N - POFUT2
Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with POFUT2 Antibody (Center) Cat.-No AP53376PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
AP53376PU-N - POFUT2
Western blot analysis of POFUT2 Antibody (Center) Cat.-No AP53376PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).
ARP45854_T100 - POFUT2
Human Stomach; POFUT2 antibody - C-terminal region (ARP45854_T100) in Human Stomach cells using Immunohistochemistry
ARP45854_T100 - POFUT2
Human HepG2; WB Suggested Antibody Titration: 2.5 ug/ml. Positive Control: HepG2. There is BioGPS gene expression data showing that POFUT2 is expressed in HepG2.; POFUT2 antibody - C-terminal region (ARP45854_T100) in Human HepG2 cells using Western Blot
ARP45855_P050 - POFUT2
Transfected 293T; WB Suggested Anti-POFUT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; POFUT2 antibody - N-terminal region (ARP45855_P050) in Transfected 293T cells using Western Blot
C15898-1 - POFUT2
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using POFUT2 Antibody. The picture on the right is treated with the synthesized peptide.
TA504972 - POFUT2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2.
TA504972 - POFUT2
HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA504972), and then analyzed by flow cytometry.
TA504982 - POFUT2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2.
TA504982 - POFUT2
HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA504982), and then analyzed by flow cytometry.
TA504983 - POFUT2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2.
TA505004 - POFUT2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2.
TA505004 - POFUT2
HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA505004), and then analyzed by flow cytometry.
TP309505 - POFUT2
TA338933 - POFUT2
WB Suggested Antibody Titration: 2.5 ug/ml; Positive Control: HepG2.There is BioGPS gene expression data showing that POFUT2 is expressed in HepG2
TA338933 - POFUT2
Human Stomach
TA338934 - POFUT2
WB Suggested Anti-POFUT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POFUT2 antibody
  POFUT2 mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2. Mouse IgG2a OTI3A12 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

POFUT2 antibody
  POFUT2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2. Mouse IgG2a OTI1A6 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

POFUT2 antibody
  POFUT2 mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2. Mouse IgG2a OTI3C12 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

POFUT2 antibody
  POFUT2 mouse monoclonal antibody, clone OTI5H1 (formerly 5H1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2. Mouse IgG1 OTI5H1 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

POFUT2 (Center) antibody
Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with POFUT2 Antibody (Center) Cat.-No AP53376PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POFUT2 (transcript variant 3)
  Recombinant protein of human protein O-fucosyltransferase 2 (POFUT2), transcript variant 3  
POFUT2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

POFUT2 overexpression lysate
POFUT2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

POFUT2 overexpression lysate
POFUT2 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pofut2 (GFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pofut2 (GFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (2)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (5)
  4. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (4)
  2. Polyclonal (1)
Target
  1. POFUT2 (6)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (5)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Flow cytometry (F) (3)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (1)
Host
  1. Mouse (4)
  2. Rabbit (1)
Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Flow cytometry (F) (3)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn