POGK antibody
Principal name
POGK antibody
Alternative names for POGK antibody
KIAA1513, LST003, SLTP003, Pogo transposable element with KRAB domain
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Luminex (LMNX)
Background of POGK antibody
- Kit Component:
- - KN222119G1, POGK gRNA vector 1 in pCas-Guide vector
- KN222119G2, POGK gRNA vector 2 in pCas-Guide vector
- KN222119D, donor vector containing left and right homologous arms and RFP-BSD functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
Cheung, C.L., et al. Hum. Mol. Genet. 18(4):679-687(2009)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Greenhalf, W., et al. Yeast 15(13):1307-1321(1999)
Immunofluorescence of monoclonal antibody to POGK on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POGK expression in transfected 293T cell line by POGK monoclonal antibody (M01), clone 1A9.Lane 1: POGK transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.
POGK monoclonal antibody (M01), clone 1A9 Western Blot analysis of POGK expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged POGK is approximately 1ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to POGK on HeLa cell. [antibody concentration 15 ug/ml]
Western Blot analysis of POGK expression in transfected 293T cell line (H00057645-T01) by POGK MaxPab polyclonal antibody.Lane 1: POGK transfected lysate(66.99 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POGK expression in transfected 293T cell line (H00057645-T02) by POGK MaxPab polyclonal antibody.Lane 1: POGK transfected lysate(69.40 KDa).Lane 2: Non-transfected lysate.
Human 293T; WB Suggested Anti-POGK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. POGK is supported by BioGPS gene expression data to be expressed in HEK293T.; POGK antibody - middle region (ARP39288_P050) in Human 293T cells using Western Blot
Human THP-1; WB Suggested Anti-POGK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; POGK antibody - N-terminal region (ARP39287_P050) in Human THP-1 cells using Western Blot
Western blot analysis of POGK Antibody (N-term) Cat.-No AP53377PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the POGK antibody detected the POGK protein (arrow).
Anti-POGK mouse monoclonal antibody (TA505548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POGK(RC222119).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
HEK293T cells transfected with either RC222119 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POGK antibody(TA505548), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505607)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POGK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505608)
Anti-POGK mouse monoclonal antibody (TA505608) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POGK(RC222119).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-POGK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505609)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505638)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POGK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POGK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505670)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POGK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000
POGK Elisa with 2D5 Capture (TA600350) and 6D5 Detection (TA700362) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Elisa with 2D5 Capture (TA600350) and 3E2 Detection (TA700350) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Elisa with 2D5 Capture (TA600350) and 3D2 Detection (TA700363) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 3D2 Detection (TA700363) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 6D5 Detection (TA700362) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 3E2 Detection (TA700350) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Elisa with 2D5 Capture (TA600350) and 3E2 Detection (TA700350) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 3E2 Detection (TA700350) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Elisa with 2D5 Capture (TA600350) and 6D5 Detection (TA700362) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 6D5 Detection (TA700362) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Elisa with 2D5 Capture (TA600350) and 3D2 Detection (TA700363) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
POGK Luminex Elisa with 2D5 Capture (TA600350) and 3D2 Detection (TA700363) Antibodies. Substrate used: Recombinant Human POGK (TP322119)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505608 | POGK antibody |
||||||
| POGK mouse monoclonal antibody, clone OTI4C8 (formerly 4C8) | |||||||
 |
Mouse | IgG2a | OTI4C8 | Purified | Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
||||||
| TA505548 | POGK antibody |
||||||
| POGK mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) | |||||||
 |
Mouse | IgG1 | OTI2D5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
