POLD3 antibody
Principal name
POLD3 antibody
Alternative names for POLD3 antibody
DNA polymerase delta subunit 3, KIAA0039, DNA polymerase delta subunit p66
Gene ID
10714 (POLD3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of POLD3 antibody
- Kit Component:
- - KN223072G1, POLD3 gRNA vector 1 in pCas-Guide vector
- KN223072G2, POLD3 gRNA vector 2 in pCas-Guide vector
- KN223072D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Baranovskiy, A.G., et al. Cell Cycle 7(19):3026-3036(2008)
Baranovskiy, A.G., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (PT 9), 822-824 (2008)
Lemmens, L., et al. Biochem. Biophys. Res. Commun. 367(2):264-270(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).
Immunofluorescence of monoclonal antibody to POLD3 on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged POLD3 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of POLD3 expression in transfected 293T cell line (H00010714-T01) by POLD3 MaxPab polyclonal antibody.Lane 1: POLD3 transfected lysate(51.40 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of POLD3 Antibody (C-term) Cat.-No AP53378PU-N in mouse spleen tissue lysates (35ug/lane). This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).
Western blot analysis of POLD3 Antibody (C-term) Cat.-No AP53378PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).
Western blot analysis of DNA Polymerase delta 3 expression in HeLa (A); MCF7 (B); Jurkat (C); mouse brain (D) whole cell lysates.
Western blot analysis of extracts from LOVO cells, HepG2 cells and mouse brain cells, using POLD3 antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315095 | POLD3 antibody |
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| Rabbit polyclonal anti-POLD3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP53378PU-N | POLD3 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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