POLE1 antibody
Principal name
POLE1 antibody
Alternative names for POLE1 antibody
DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, POLE
Gene ID
5426 (POLE)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of POLE1 antibody
- Kit Component:
- - KN224742G1, POLE gRNA vector 1 in pCas-Guide vector
- KN224742G2, POLE gRNA vector 2 in pCas-Guide vector
- KN224742D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to POLE on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POLE expression in transfected 293T cell line (H00005426-T01) by POLE MaxPab polyclonal antibody.Lane 1: POLE transfected lysate(40.81 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to POLE on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POLE expression in transfected 293T cell line (H00005426-T01) by POLE MaxPab polyclonal antibody.Lane 1: POLE transfected lysate(40.81 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation/Western blot analysis of POLE expression in 293 cells. Immunoprecipitation ( denatured verified ) using 2 µl/mg protein lysate. Use Protein G.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLE1 antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314359 | POLE1 antibody |
||||||
| Rabbit polyclonal anti-POLE antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN224742 | POLE - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224742BN | POLE - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC224742L1V | Lenti ORF particles, POLE (Myc-DDK tagged) - Human polymerase (DNA directed), epsilon (POLE), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,420.00
|
|
| OriGene Technologies, Inc. | ||
