POLR1C antibody
Principal name
POLR1C antibody
Alternative names for POLR1C antibody
RNA polymerases I and III subunit AC1, RPA40, RPA39, RPC40, POLR1E, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide
Gene ID
9533 (POLR1C)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of POLR1C antibody
- Kit Component:
- - KN203486G1, POLR1C gRNA vector 1 in pCas-Guide vector
- KN203486G2, POLR1C gRNA vector 2 in pCas-Guide vector
- KN203486D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hu P., et al (2002). Mol. Cell. Biol. 22:8044-8055Dauwerse J.G., et al (2011). Nat. Genet. 43:20-22
Immunoperoxidase of monoclonal antibody to POLR1C on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to POLR1C on HeLa cell. [antibody concentration 7 ug/ml]
POLR1C monoclonal antibody (M01), clone 3A5-H2 Western Blot analysis of POLR1C expression in K-562 ( Cat # L009V1 ).
POLR1C monoclonal antibody (M03), clone 2E11 Western Blot analysis of POLR1C expression in HeLa ( Cat # L013V1 ).
Immunoperoxidase of monoclonal antibody to POLR1C on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 0.6 ug/ml]
POLR1C monoclonal antibody (M05), clone M1 Western Blot analysis of POLR1C expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged POLR1C is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of POLR1C expression in transfected 293T cell line (H00009533-T01) by POLR1C MaxPab polyclonal antibody.Lane 1: POLR1C transfected lysate(38.06 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of anti-POLR1C Antibody (C-term) (Cat.#AP12496PU-N) in A2058 cell line lysates (35ug/lane). POLR1C (arrow) was detected using the purified Pab.
Western blot analysis of POLR1C expression in SW480 (A); K562 (B); NIH3T3 (C) whole cell lysates.
Predicted band size: 39 kDa . Positive control: K562 and SW480 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: SW480 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Nucleus. Positive control: Human spleen tissue. Recommended dilution: 1/50-200 The image is immunohistochemistry of paraffin-embedded Human spleen tissue using POLR1C antibody at dilution 1/50
Host: Rabbit; Target Name: POLR1C; Sample Tissue: Jurkat Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303486 | POLR1C (transcript variant 1) |
||||
| Recombinant protein of human polymerase (RNA) I polypeptide C, 30kDa (POLR1C), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
