POLR1E antibody
Principal name
POLR1E antibody
Alternative names for POLR1E antibody
RNA polymerase I subunit A49, PAF53, PRAF1, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit E,RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of POLR1E antibody
- Kit Component:
- - KN313596G1, Polr1e gRNA vector 1 in pCas-Guide vector
- KN313596G2, Polr1e gRNA vector 2 in pCas-Guide vector
- KN313596D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to PRAF1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POLR1E expression in transfected 293T cell line (H00064425-T01) by POLR1E MaxPab polyclonal antibody.Lane 1: PRAF1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
POLR1E MaxPab polyclonal antibody. Western Blot analysis of POLR1E expression in U-2 OS.
Immunofluorescence of purified MaxPab antibody to PRAF1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of POLR1E expression in transfected 293T cell line (H00064425-T01) by POLR1E MaxPab polyclonal antibody.Lane 1: PRAF1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
POLR1E MaxPab polyclonal antibody. Western Blot analysis of POLR1E expression in U-2 OS.
Lanes: Lane1: 50 ug mouse heptoma cell lysate; Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1:5000; Gene Name: PORL1E; Submitted by: Eekjoong Park, UCSD;
WB Suggested Anti-POLR1E Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304811 | POLR1E |
||||
| Recombinant protein of human polymerase (RNA) I polypeptide E, 53kDa (POLR1E) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207725L2V | Lenti ORF particles, Polr1e (GFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR207725L1V | Lenti ORF particles, Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||